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University of Guelph Canada (2000)

Bio-engineering alfalfa (Medicago sativa L.) to accumulate fructans

Bernal, Libia Maritza

Titre : Bio-engineering alfalfa (Medicago sativa L.) to accumulate fructans

Auteur : Bernal, Libia Maritza

Université de soutenance : University of Guelph Canada

Grade : Master of Science 2000

Alfalfa (Medicago sativa L.) clone N4-4-2 was bio-engineered for the synthesis of fructans in order to generate genetic variability to improve its winter hardiness, yield stability, and forage quaiity. The sa& gene from Bacillus amyloliquefaciens encding levansucrase was expresseci and targeted to the cytosol. T, genotypes accumulated fructan in crown tissue but winter suwival was reduced. Three expression vectors were constructed to deliver the levansucrase protein to the vacuole. Two constitutive promoters (CaMV 35s and Super promoter) or a mot specific promoter (MasT) were used. A group of 25 To plants per constnict were produced by Agrobacteriumrnediated transformation. PCR and Southem hybridisation confirmed insertion of the sacB gene. In a field trial established at Elora, Ontario, in 1999, transgenic alfalfa plants exhibited significantly higher dry matter accumulation than the wild-type. Cmwn tissue accurnulated fructan up to 13 mq(g dw and also contained more starch than the witd type.

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Page publiée le 29 mars 2010, mise à jour le 14 septembre 2019