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Université de Mongolie-Intérieure (2020)

Soil Microbial Diversity and Characteristics of Reclaimed Vegetation in Coal Mine Subsidence Area of Semi-arid Grassland


Titre : Soil Microbial Diversity and Characteristics of Reclaimed Vegetation in Coal Mine Subsidence Area of Semi-arid Grassland

Auteur : 郭洋楠;

Grade : Doctoral Dissertation 2020

Université : Université de Mongolie-Intérieure

Résumé partiel
The Shenfu-Dongsheng mining area is rich in coal resources and is the world’s first modern production base with a capacity of 200 million tons of coal.The mining area is located in the arid and semi-arid ecologically fragile areas in western China.Due to large-scale and high-intensity coal mining activities,a series of ecological and environmental problems is inclined to occur,such as soil structure destruction,loss of soil moisture and nutrients,root system damage of vegetation,and reduction of plant and microbial diversity.Artificial vegetation construction by the use of different types of plants in the coal subsidence area is an important measure for ecological restoration in the mining area.While our knowledge of the secondary disturbance of the ecological control measures(transplanting and afforestation project)to the regional ecological environment in the coal subsidence area,especially to the diversity of environmental soil microorganism and the soil factors is insufficient.With the Shendong coal mine subsidence area and its artificial planting area in the semi-arid steppe area as the research areas,the regional plant communities are investigated and the soil physical and chemical properties were determined,and then soil microbial diversity and ecological distribution characteristics under thedisturbance of mining subsidence and afforestation were discussed.The main conclusions of this study are as follows :(1)there are 4 families,9 genera and 10 species of native plants in the Shendong coal mine area and the native plants are primarily perennial herbs with a few biennial herbs.Leguminous plants and gramineous plants are the major native plant species,accounting for 60% of the total native plant species.There is no significant difference in the plant diversity of the native herbaceous vegetation community under the reclaimed vegetation,there is no significant difference in the Shannon Wiener index and Simpson index of the herb community(P>0.05),and the natural restoration biomass is significantly increased(P<0.05).(2)In the coal mining induced subsidence areas,For the actinomycetes,their number decreased significantly(significance level<0.05)and the genetic diversity indexes,including Sobs,Shannon,Ace and Chao1,decreased significantly(significance level<0.05).For the fungal,the genetic diversity indexes,including Sobs,Ace and Chao1,decreased significantly(significance level<0.05).The species richness of AM fungal decreased significantly(significance level<0.05)and AM fungal genetic diversity indexes,including Sobs,Shannon and Chao1,decreased significantly(significance level<0.05).Total carbon content increased significantly(significance level<0.05) ;Urease content decreased significantly(significance level<0.05).The subsidence lowered the genetic diversity of soil microorganisms more markedly.(3)5664 bacterial OTUs were detected in the study area and can be annotatedto 40 phyla,99 classes,188 families,357 orders,626 genera,1329 species.Most of them belong to Actinobacteria,Proteobacteria,Chloroflexic and Acidobacteria.The impact of transplantation types on the total quantity and abundance variation range of soil bacterial is small.2038 fungal OTUs were detected and can be annotated to7 phylum,31 phyla,88 classes,192 families,408 genera and 671 species.

Mots clés : semi-arid; coal mining subsidence area; soil microorganisms; high-throughput sequencing; soil factors; reclaimed vegetation;

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Page publiée le 22 octobre 2020, mise à jour le 26 octobre 2021