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Accueil du site → Doctorat → États-Unis → 2020 → Introducing an Indigenous Non-toxigenic Aspergillus flavus Strain Isolated from Iraqi Corn Grains as a Bio-Control Agent to Reduce Aflatoxin Contamination in Corn Grains

University of Arkansas (2020)

Introducing an Indigenous Non-toxigenic Aspergillus flavus Strain Isolated from Iraqi Corn Grains as a Bio-Control Agent to Reduce Aflatoxin Contamination in Corn Grains

Ali Almatakeez,

Titre : Introducing an Indigenous Non-toxigenic Aspergillus flavus Strain Isolated from Iraqi Corn Grains as a Bio-Control Agent to Reduce Aflatoxin Contamination in Corn Grains

Auteur : Ali Almatakeez,

Université de soutenance : University of Arkansas

Grade : Doctoral of Philosophy in Cell and Molecular Biology 2020

Résumé
Mycotoxin contamination of cereal crops, such as maize and sorghum, is a global concern because of the potential health effects on humans and animals. Although substantial research has been conducting regarding mycotoxin prevention and mitigation, little information is available about the association of mycotoxin-producing fungi with corn and sorghum grain in Iraq. Identifying and refining indigenous atoxigenic strains to reduce mycotoxin contamination of maize and sorghum has the potential to enhance the nutritional value of these grains while reducing economic losses. However, to our knowledge, this tactic has not yet been adopted by agricultural authorities and farmers in Iraq. To survey mycotoxigenic fungi associated with corn and sorghum grain in Iraq and identify potential biological control agents ‘customized’ for Iraqi production conditions, a collection of corn and sorghum grain samples were imported from Iraq. DNA-based diagnostic analyses were integrated with morphology-dependent methods to identify seedborne mycoflora in corn and sorghum samples collected from different regions in Iraq (north, central, and south). The most common fungal genera, i.e., Fusarium spp., Alternaria spp., Chaetomium spp., Penicillium spp., and Aspergillus spp. were found frequently in both kinds of grains. However, Exserohilum, Anthracosystis, Bipolaris, Sporisorium, Curvularia, Sarocladium, Humicola, Byssochlayms, and Stenocarpella were found associated with some samples, which is the first such reporting of these genera associated with corn in Iraq. Moreover, species borders of Aspergillus spp. and Fusarium spp. were delimited successfully through multi-locus sequence typing analysis of four conserved genes within each genera (ITS, B-tub, CaM, and RPB-2 for Aspergillus spp. and EF-1ɑ, CaM, and H3 for Fusarium spp.).

Mots clés  : Aflatoxins, Biological control, Fumonisins, Maize grains, Mycotoxigenic fungi, Pathogenic fungi

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Page publiée le 28 octobre 2020