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Universidade Federal Rural de Pernambuco (2007)

Germinação in vitro de Parkinsonia aculeata L. : uma espécie de uso múltiplo ocorrente nas matas ciliares da caatinga.

GOMES, Maria Luiza de Oliveira

Titre : Germinação in vitro de Parkinsonia aculeata L. : uma espécie de uso múltiplo ocorrente nas matas ciliares da caatinga.

Auteur : GOMES, Maria Luiza de Oliveira

Université de soutenance : Universidade Federal Rural de Pernambuco

Grade : Mestre em Botanica 2007

Résumé
The ecological information and forms of use of the Turk (Parkinsonia aculeata L.) propose the species as good component for the agroforests systems in caatinga areas. The agroforests systems can assure source of income and sustainability, by the use of species for the ends of use bees, woodman, feeded, medicinal, ornamental and still in the recovery of the ciliary forests of the caatinga, emphasizing the importance of the hidrics resources and the biodiversity conservation, serving as warranty for the rural producers and communities of the caatinga. The development of the agroforests systems initially demands a significant amount of seedlings. The in vitro germination of the threatened species of extinction or that should be introduced in systems of native vegetation assures the genetic variability of those species and make available seedling free from microorganisms that can be maintained as matrix and serve as source of primary explants in the micropropagation process. The in vitro multiplication, or micropropagation, assures a production in commercial scale of seedlings with high quality phytosanitary and nutritional. The objective of this work was, therefore, to develop a protocol of in vitro germination of P. aculeata guaranteeing the obtaining of clean seedling for micropropagation. The experiment was driven at the Laboratório de Cultura de Tecidos Vegetais of the UFRPE. The seeds were coming of the collection of several fruits in matrix of P. aculeata located in the district of Betânia, semi-arid area of Pernambuco. The fruits were benefited and the selected seeds separated in lots of 100 units and scarified with sand to overcome the dormancy. Soon afterwards were submitted to the disinfestations with distilled water, following by wash in solution with 3 drops of Tween-20 in 1000ml of distilled water and three rinsed with distilled water. Soon afterwards, the seeds were immersed in alcohol to 70% for 1 minute, following by different times of soak in sodium hypochlorite (NaOCl) 0,6% before the germination and, finally, rinsed for five times with water distilled sterilized, in laminar flow camera. The seeds were, then, inoculated in the medium MS and ½ MS in agreement with the treatments to proceed : T1 = MS-0min ; T2 = MS-5min ; T3 =MS-10min ; T4 = ½MS-0min ; T5 = ½MS-5min and T6 = ½MS-10min. The in vitro germination of seeds of Parkinsonia aculeata does not depend on the ionic strength of the MS medium. The asepsis of seeds of P. aculeata with 0,6% of sodium hypochlorite (NaOCl) for 10 minutes inhibits the germination, favors the oxidation and reduces the percentage of emerged plants.

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