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Ankara University (2018)

DETERMINATION OF MORPHOLOGICAL-ANATOMICAL, PHYSIOLOGICAL AND GENE EXPRESSION CHANGES OF SOME OLIVE CULTIVARS UNDER WATER DEFICIENT STRESS

GULER Zerbab

Titre : DETERMINATION OF MORPHOLOGICAL-ANATOMICAL, PHYSIOLOGICAL AND GENE EXPRESSION CHANGES OF SOME OLIVE CULTIVARS UNDER WATER DEFICIENT STRESS

Bazı zeytin çeşitlerinde su noksanlığı stresinde morfolojik- anatomik, fizyolojik ve gen ifadesi değişimlerin belirlenmesi

Auteur : GULER Zerbab

Université de soutenance : Ankara University

Grade : Doctor of Philosophy (PhD) 2018

Résumé
Drought is thought to be one of the most important limiting factors in the cultivation of olive (Olea europaea L.) as well as in many plant species. For this reason, elucidation of plant defense mechanisms against environmental stress factors are a very important issue. In this context, it was aimed to investigate the changes in anatomical, morphological, physiological level and also ingene expressions in four commercial olive cultivars (Ayvalik, Domat, Manzanilla, Kilis Yaglık). 2-years-old olive seedlings with their own roots were not irrigated for 45 days and the stress plants were re-irrigated for 45 days after drought stress and the development of olive cultivars after drought stress was investigated. Each cultivar of leaf tissue each cultivar were collected biweekly and the number and diameter of stomas, number and diameter of trichome, leaf relative water content, leaf water potential were analyzed and also real-time PCR exprements were conducted. For olive cultivars, the number of trichome and leaf water potential changes per unit leaf area were found statistically significant under drought stress condition. Therefore, number of trichome per unit leaf area and leaf water potential were important parameters for the determination of drought stress. At the same time,DHN1, DHN6, OESDHN genes were identified as useful workers for detection of tolerance capability of olive cultivar against to drought stress conditions according to the results obtained by analysis.

Mots clés : Olea europaea L., water deficient stress, morphology-anatomy, physiology, dehidrin (DHN) genes

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