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Ankara University (2020)

GENOME-WIDE IDENTIFICATION OF DROUGHT RESPONSIVE HSP70 IN COMMON BEAN

TANRISEVEN Mehmet

Titre : GENOME-WIDE IDENTIFICATION OF DROUGHT RESPONSIVE HSP70 IN COMMON BEAN

FASULYE BĠTKĠSĠNDE KURAKLIĞA DUYARLI HSP70 GENLERĠNĠN TÜM GENOMDA TANIMLANMASI

Auteur : TANRISEVEN Mehmet

Université de soutenance : Ankara University

Grade : Master Thesis 2020

Résumé
Beans are one of the most cultivated legume species in the world and Turkey. Abiotic stresses, affect production in beans like many plants. Plants have developed various defense mechanisms against abiotic stresses. In recent years, the use of in-silico tools to clarify the defense mechanisms of plants against environmental factors has gained importance.

In this thesis, it was aimed to identify HSP70 genes susceptible to drought stress in common bean by using various in-silico methods. 24 PvHSP70 genes were identified and their localization on the genome, three-dimensional protein structures of exon-intron regions were also determined. Nine of the PvHSP70 genes which play roles in drought stress were selected based on RNA-Seq data. Two different common bean varieties known to be drought stress resistant (Yakutiye) and susceptible (Zülbiye) were exposed to 100 mM drought stress (PEG) for 24 hours. Samples were taken from the root and leaf tissues of both varieties and RNA isolation, cDNA synthesis and Real-Time PCR analysis via Light Cycler Nano instrument were performed. PvHSP70-1, -20 and -24 genes were found to be higher in the leaf tissues of Zülbiye than Yakutiye. The decrease in the mRNA level of PvHSP70-12 gene in Yakutiye variety was found to be higher than Zulbiye. There was a significant decrease in the mRNA level of the selected genes except for PvHSP70-20 gene in the root tissues of the Zülbiye. Additionaly, the decreases in PvHSP70-1, -4, -6 and -20 genes of Yakutiye were found to be statistically significant. It is thought that mRNA fold changes of HSP70 genes under drought stress may be used in various breeding studies in biotechnology field and will shed light on molecular marker studies.

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Page publiée le 20 décembre 2020