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University of Johannesburg (2019)

The antimicrobial activity of secondary metabolites produced by bacterial endophytes isolated from Pellaea calomelanos

Mahlangu, Siphiwe Godfrey

Titre : The antimicrobial activity of secondary metabolites produced by bacterial endophytes isolated from Pellaea calomelanos

Auteur : Mahlangu, Siphiwe Godfrey

Université de soutenance : University of Johannesburg

Grade : Master of Science (MS) Biotechnology 2019

Résumé partiel
Medicinal plants have been used worldwide as traditional remedies for the treatment of various diseases such as asthma, skin disorders, cardiovascular diseases, gastrointestinal symptoms, respiratory and urinary problems. These plants have shown to synthesize biologically active compounds that can be applied in drug discovery and other industries. The use of medicinal plants has led to deforestation and extinction of plant species ; therefore, this has compelled scientists in search for new alternates for drug discovery and development. One of these are endophytes, which are microorganisms associated symbiotically with medicinal plants. Endophytes produce bioactive compounds similar to their plant host ; in addition, endophytes produce various bioactive secondary metabolites some of which protect the plant host from pathogens and enhance growth potential of the plant host. This research study aimed at isolating and identifying bacterial endophytes from Pellaea calomelanos, a medicinal plant used for the treatment of headaches, chest and head colds, asthma, nasal and mouth ulcers, and to further evaluate the antibacterial activity of crude extracts produced by the plant and its bacterial endophytes. Six putative bacterial endophytes were isolated from surface sterilized leaves of P. calomelanos and identified using morphological and phenotypic data, the identification was confirmed by the 16S rRNA gene sequence, viz Arthrobacter sp. MHSD1 (MF613647), Pantoea sp. MHSD2 (MF613648), Bacillus infantis strain MHSD3 (MF613649), Pantoea sp. strain MHSD4 (MF613650), Pantoea ananatis strain MHSD5 (MF613651) and Pantoea sp. MHSD6 (MF613652)

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Page publiée le 31 décembre 2020