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Accueil du site → Master → Afrique du Sud → 2018 → In vitro assessment of selected ethno-medicinal plants as potential alternatives for the control of gastrointestinal nematodes in sheep and goats.

Mhlongo, Lindokuhle Christopher.

In vitro assessment of selected ethno-medicinal plants as potential alternatives for the control of gastrointestinal nematodes in sheep and goats.

Mhlongo, Lindokuhle Christopher.

Titre : In vitro assessment of selected ethno-medicinal plants as potential alternatives for the control of gastrointestinal nematodes in sheep and goats.

Auteur : Mhlongo, Lindokuhle Christopher.

Université de soutenance : University of KwaZulu-Natal

Grade : Master of Science in Agriculture (Animal Science) 2018

Résumé partiel
Commercial anthelmintics are becoming ineffective against gastrointestinal nematodes (GIN) of ruminants due to development of resistant parasites. Research is exploiting anthelmintic ethno-medicinal plants for an alternative remedy. This study assessed the in vitro : (1) dose activity at different concentrations, (2) combined synergistic activity of ethanolic crude plant extracts on mixed GIN of sheep and goats ; and (3) cytotoxic activity of these extracts on kidney vero cells. During assessment of in vitro dose activity, faecal samples of sheep and goats that were grazing on contaminated pasture were collected, cultured (12 days) to L3 larvae stage, and treated with 40, 20, 10, 5, 2.5, 1.25 and 0.25% v/v of Allium cepa, Ananas comosus, Bidens pilosa, Carica papaya, Crinium macowanii, Gunnera perpensa, Nicotiana tabacum, Ricinus communis, Sarcosterma viminale, Trema orientalis, Urtica dioica, Vernonia amygdalina, Zanthozylum capense, Zingiber officinale, Zizyphus mucronata and Aloe vanbalenii extracts. Larvae were subjected to Baermann technique for isolation and later observed under a microscope (10x objective). During the assessment of synergism at 1.25% v/v concentration (1:1), 28 crude plant extract combinations from eight (8) mostly edible plants namely : Allium cepa, Ananas comosus, Bidens pilosa, Carica papaya, Vernonia amygdalina, Zingiber officinale, Aloe vanbalenii and Nicotiana tabacum (inedible) were tested for their synergistic activity. The simple and Webb’s fractional product method were used to compute interactions of crude plant extract combinations.

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Page publiée le 17 janvier 2021