Informations et ressources scientifiques
sur le développement des zones arides et semi-arides

Accueil du site → Master → Afrique du Sud → 2019 → Bioavailability of nutrients in a diet enriched with moringa oleifera lam. Leaves using wistar rats

University of Fort Hare (2019)

Bioavailability of nutrients in a diet enriched with moringa oleifera lam. Leaves using wistar rats

Mhlomi, Yanga Nonelela

Titre : Bioavailability of nutrients in a diet enriched with moringa oleifera lam. Leaves using wistar rats

Auteur : Mhlomi, Yanga Nonelela

Université de soutenance : University of Fort Hare

Grade : Masters in Botany 2019

Résumé partiel
This study investigated the nutritional composition and bioavailability of nutrients in Moringa oleifera leaf meal from South African ecotype. Nutritional evaluation (proximate, mineral, vitamins and antinutrients, amino acids and fatty acids analyses) was performed using AOAC, ICP-OES and GC+MS techniques respectively. Bioavailability of nutrients involved formulation of diets deficient in minerals and vitamins ; and proteins supplemented with Moringa oleifera leaf meal (MOLM) at 0, 3, 5 and 10% respectively. Growth performance, hematological, biochemical and histopathological evaluations were used to assess the effect of MOLM supplementation. Proximate and mineral evaluation revealed that the leaves were rich in protein (28.72%) and carbohydrate (28.84%), calcium content was (1603.33 mg/100g), potassium (1690 mg/100g), zinc (13.03 mg/100g) and iron (21.13 mg/100g). Vitamin E content was the highest (89.43 mg/100g) followed by vitamic C (24.9 mg/100g) and vitamin A (12.98 mg/100g). Fatty acids analysis detected were fifteen of which polyunsaturated fatty acid linoleic acid (56.36) and alpha-linolenic acid (687.58) were highest and behenic acid (22:0) characteristics of Moringa (4.11%) were detected. Seven of the detected fatty acids were saturated fatty acids, though relatively low, with stearic acid having the highest value. On the other hand fifteen of the amino acids, 7 were essential (valine, leucine, isoleucine, methionine, threonine and phenylalanine) were detected ; while alanine, proline, serine, aspartic acid, cysteine, glutamic acid, asparagine and tyrosine were non- essential amino acid. Moringa oleifera had low quantities of antinutrients in relation to minerals, thus nutrients will easily ne available. Amino and fatty acids profile of nutrient deficient diets supplemented with MOLM revealed that six essential amino acids were detected in the diets. However, the essential amino acid (EAA) in MOLM with the highest value was threonine (500.47±23.03mg/L), while the lowest was methionine (75.65±5.31mg/L). Leucine was highest in PD10 (234.32±27.98 mg/l) 15 and lowest in PD3. Predominant amino acid in VMD diet was glutamic acid (966.76±208.92 mg/l), while methionine was lowest (197.14±35.90). Caproic acid was the most prominent unsaturated fatty acid in the diets and was highest in the PD diet. Palmitoleic acid was highest in the control diet (8.19±0.56 mg/L) and lowest in PD3 but an increase was observed as the levels of MOLM increased in the diets. Linoleic acid values obtained were significantly higher in PD diet, lower in control diet, compared to other diets. Linolenic acid (C18-3n6) was higher in PD10 (63.48±3.84 mg/L) compared to the control diet (6.05±0.14 mg/L).

Présentation (SEALS)

Version intégrale

Page publiée le 18 janvier 2021