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Accueil du site → Doctorat → Australie → 2016 → Application of high-throughput sequencing technologies to determine the molecular basis of drought tolerance in chickpea (Cicer arietinum L.)

RMIT University (2016)

Application of high-throughput sequencing technologies to determine the molecular basis of drought tolerance in chickpea (Cicer arietinum L.)

Bhaskarla Vijay

Titre : Application of high-throughput sequencing technologies to determine the molecular basis of drought tolerance in chickpea (Cicer arietinum L.)

Auteur : Bhaskarla Vijay

Université de soutenance : RMIT University

Grade : Doctor of Philosophy (PhD) 2016

Résumé partiel
Drought stress is one of the most important abiotic stresses, which adversely effects chickpea (Cicer arietinum L.) production across the globe. It is responsible for substantial yield loses up to 50%, which led to stagnated productivity of chickpea for the past six decades. In chickpea, terminal drought stress leads to significant reduction in the seed yield (58-95%). The deleterious effects of terminal drought stress are mainly manifested as increased flower and pod abortion, reduced pod production, and reduced seed size. For the past 20 years, root traits such as deep and profuse rooting system have been proposed as a main breeding target for improving drought tolerance in chickpea. Due to the complexity of drought stress, traditional breeding approaches have been largely unsuccessful in exploiting root traits for developing tolerant chickpea cultivars. Drought tolerance is a complex quantitative trait, which is influenced by number of genetic and environmental interactions. It is mainly controlled by the several drought responsive genes or gene networks and shows genotypic divergence depending on the plant phenology. In addition, plants show genotype-, tissue- and stage-specific variations during their response to drought stress. Hence, understanding the physiological and genetic basis of drought stress in a genotype-, tissue- and stage-specific manner is essential to decipher the complexity of drought stress. Till date, no study was focused on deciphering the molecular mechanisms that underlie root and reproductive growth during drought stress in chickpea. Therefore, this study was designed to employ RNA-Sequencing (RNA-Seq) for investigating genome-wide transcriptome changes in the roots and reproductive tissues during different developmental stages in response to drought stress. Further, the study also investigated the role of drought responsive microRNAs during reproductive development. This will improve an overall understanding of molecular mechanisms that control root and reproductive development during drought stress.

Mots clés : Chickpea RNA Sequencing Computational biology Drought tolerance micro RNAs Plant physiology Bioinformatics Plant Physiology Gene Expression (incl. Microarray and other genome-wide approaches)

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