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Master
Espagne
Aprovechamiento de subproductos de la oliva : Uso del hidroxitirosol para mejorar la actividad antioxidante y la estabilización de la vitamina C en zumos.
Titre : Aprovechamiento de subproductos de la oliva : Uso del hidroxitirosol para mejorar la actividad antioxidante y la estabilización de la vitamina C en zumos.
Auteur : Bouchal Yougourthane
Université de soutenance : Université de Cordoba
Le programme du Master est organisé conjointement par Universidad de Córdoba (UCO), Consejería de Agricultura, Pesca y Medio Ambiente de la Junta de Andalucía (CAP), Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción Ecológica (IFAPA), Centre International de Hautes Etudes Agronomiques Méditerranéennes (CIHEAM) à travers l’Institut Agronomique Méditerranéen de Zaragoza (IAMZ), Consejo Superior de Investigaciones Científicas (CSIC) et Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
Grade : Master Universitaire en Oliviculture et Oléotechnie 2019
Résumé en Espagnol – Anglais
Hydroxytyrosol (HT) is one of the main natural phenolic compounds present in olives, virgin olive
oil, table olives and residues generated during olive processing. It is of great interest due to its
antioxidant properties and its role in the prevention of various diseases associated with
oxidative stress (cancer, type 2 diabetes, cardiovascular disease).
Keeping in mind the antioxidant properties of HT, the evolution of vitamin C within standard
solutions has been studied, as well as within a food matrix rich in this vitamin C as a strawberry
puree. These matrices were enriched with HT at different concentrations. In view of the
sensitivity of vitamin C and its rapid oxidation, the colorimetric method was chosen faster and
simpler than the HPLC method for determining the two bioactive forms, and its evolution over
time was studied. Who reveals a more important degradation of vitamin C at room temperature
than at 4 ºC in the case of standard solution. While for strawberry puree a rapid diminution of
vitamin C (AA+ ADHA) at 4ºC storage is observed, in the control assay (without hydroxytyrosol),
On the other hand, if we analyze the effect of HT on vitamin C, HT does not protect the
degradation of vitamin C at any of the concentration and temperature conditions used for
samples in standard solution. Observing that at a low concentration of HT (0.05 mg/mL) in the
case of strawberry purée, it prevents the decrease of vitamin C content. In addition, we studied
the interaction between HT and vitamin C on antioxidant activity, DPPH and FRAP in strawberry
purée and the effects (synergic, additive, or antagonistic) which can exist between strawberry
purée and HT using combination index using the compuSyn software. Revealing values around
1, indicating an additive effect of the interaction.
Page publiée le 26 février 2021