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Accueil du site → Doctorat → Espagne → 2019 → The effect of increasing temperature on olive trees (Olea europaea L. subsp. europaea) biology : An integrated morphological, phenological and biomolecular study

Universidad de Córdoba (2019)

The effect of increasing temperature on olive trees (Olea europaea L. subsp. europaea) biology : An integrated morphological, phenological and biomolecular study

Sánchez-Lucas, Rosa

Titre : The effect of increasing temperature on olive trees (Olea europaea L. subsp. europaea) biology : An integrated morphological, phenological and biomolecular study

Estudio del efecto del aumento de la temperatura asociado a las condiciones del cambio climático sobre el olivo (Olea europaea L.), mediante el uso integrado de técnicas de fisiología y bioquímica y bioquímica clásica, proteómica, metabolómica y transcriptómica

Auteur : Sánchez-Lucas, Rosa

Université de soutenance : Universidad de Córdoba

Grade : Doctoral Tesis 2019

Résumé
This Doctoral Thesis is focused on olive tree (Olea europaea L. subsp. europaea), the dominant tree crop over large areas of the Western Mediterranean Basin, with a production of 20,872,788 tonnes in 2018. Olive oil is appreciated worldwide because of its chemical composition, nutraceutical value, and organoleptic properties. Like most crops, the olive tree is facing important problems and challenges related to adverse environmental conditions, either biotic or abiotic ones, a situation that can be worsened in a climate change scenario. Hence, understanding the physiological and molecular basis/mechanism of olive tree adaptability and resilience to different environmental stresses, especially to high temperature, is crucial for predicting the consequences of climate warming, for implementing agricultural practices, designing classic or biotechnological breeding programs, and, lately, increasing its production in order to cope with the current demand. In the present work, both the effect and the responses to an increase of 4 °C above ambient temperature have been studied at phenological, physiological and molecular,-omics, levels, in an attempt to integrate all the results in the novel Systems Biology direction. The use of the –omics techniques (transcriptomics, proteomics, and metabolomics), and its integration with physiological and phenological studies constitute the great novelty of this work. It has required an important effort in optimizing different protocols for transcriptome (RNA isolation and qRT-PCR), proteome (shotgun nLC-MS/MS Orbitrap), and metabolome (UHPLC-MS/MS Q-tof) profiling, together with computational tools for the identification and quantification of the different biomolecules, their functional classification, statistical analysis, clustering and networking. Both newly developed tools and knowledge generated will establish the basis for ongoing and future studies to obtain a better understanding of the molecular mechanisms mediating phenotypes of interest (productive, tolerant to environmental cues, nutraceutical value and the selectionof genotypes more resilient to climate warming). The manuscript has been organized and presented in nine chapters as follows : Chapter I summarises the state of the art of the topics covered in this thesis : the experimental system, objectives, hypothesis and methodologies. It starts with the description of the olive tree as a plant species and crop, its economic and cultural importance, problems and challenges related to its cultivation.

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