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Banaras Hindu University (2014)

The Functional Characterization of Calmodulin Binding Transcription Activator in response to Drought stress in plant

Pandey, Neha

Titre : The Functional Characterization of Calmodulin Binding Transcription Activator in response to Drought stress in plant

Auteur : Pandey, Neha

Université de soutenance : Banaras Hindu University

Grade : Doctor of Philosophy (PhD) 2014

Résumé partiel
During this century, water limitations are likely to increase in agriculture, and hence, the development of crop varieties with better water use efficiency (WUE) is of prime importance in agriculture. The cultivated cotton (Gossypium sp.) is one of the most important crop of the world, it is the leading producer of natural fibre used in the textile industry and is the second most important oil seed crop. Over 95% of the commercially cultivated cotton in the world is produced from Gossypium hirsutum and 2% from Gossypium herbaceum. Nearly 10 – 15% production of cotton is loss due to drought. Approximately one third of the world arable land suffers from chronically inadequate supply of water for agriculture. The drought is polygenic trait and hence one of the most complicated trait to understand at molecular level. Drought stress affects the cotton plants by limiting fibre yield and lint quality. Therefore, the term „drought tolerance‟ relates to final yield rather than to the capacity of the plant to survive in water-limited conditions. Hence, enhancing drought tolerance has been one of the key issues in the practice of cotton planting. Due to stress, leaf area, number of nodes, number of balls per plant and photosynthesis are reduced in the cotton. If an extreme drought period occurs, cotton production is greatly lowered. Numerous studies have shown root being the first sensor for water limited condition in soil. Recent research on maize yield trend in the USA clearly suggested that total yield can be vastly affected by the alteration in root architecture. Roots system holding the numerous plant functions such as soil water content, nutrient uptake, interaction with symbiotic organism, changes in nutrient availability etc. Therefore it can be confidently stated that understanding the root architecture could be relevant strategy for enhancing total plant growth and yield.

Thus our interest is to study the response of drought and understand the genetic network which controls the drought response in plants. The Gossypium herbaceum also known as desi cotton in India is generally cultivated in drought prone areas because of their inherent ability to tolerate drought, therefore, we used G. herbaceum as a model species in understanding drought tolerance. The global gene expression profiling of drought contrasting genotypes of G.herbacuem using pyrosequencing, we identified one calcium regulated transcription factor named Calmodulin Binding Transcription Activator (CAMTA). The CAMTA was found to be expressed at much higher levels in the roots of drought tolerant genotype as compared to the drought sensitive genotype. The phylogenetic analysis revealed a CG-1 homology DNA-binding domain (binding site includes MCGCGB and MCGTGT motif), three ankyrin repeats (implicated in protein-protein interaction), a putative transcriptional activation domain (an immunoglobulin-like fold involved in nonspecific DNA binding), and five putative CaM-binding motifs (called as IQ motif). Présentation et version intégrale (Shodhganga)

Page publiée le 14 février 2021