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Banaras Hindu University (2016)

Molecular and biochemical characterization of drought response in muskmelon Cucumis melo L

Ansari, Waquar Akhter

Titre : Molecular and biochemical characterization of drought response in muskmelon Cucumis melo L

Auteur : Ansari, Waquar Akhter

Université de soutenance : Banaras Hindu University

Grade : Doctor of Philosophy (PhD) in Biotechnology 2016

Résumé partiel
The present investigation, “Molecular and biochemical characterization of drought response in muskmelon (Cucumis melo L.)” was done with objective given below.  To standardize a field screening technique and evaluation of muskmelon genotypes for drought tolerance.  To evaluate the genetic diversity in muskmelon based on SSR markers and morphological traits under controlled and water-deficit condition.  To study the alteration in free radical generation and antioxidant defense machinery of muskmelon (Cucumis melo L.) genotypes in response to water-deficit.  To study the physiological adaptation, changes in antioxidant enzyme activity and gene expression analysis of two muskmelon genotypes under progressive waterdeficit condition.  To compare the muskmelon leaf proteome when exposed to increasing water-deficit condition.

During the growing season, plants face many unacceptable environmental conditions, which include biotic factors and abiotic factors like drought, heat, salinity, flooding, heavy metals, and change in soil pH. These stresses have negative impact on plants survival, biomass production and estimated up to 82% loss of final yield. Drought stress is one of the most critical reasons of crop loss worldwide. It has been expected that the highest yield reduction is caused by drought stress. Drought stress is a serious threat to agriculture that results in decreased crop yield. Drought stress leads to a series of morphological, physiological, biochemical and molecular changes that adversely affect plant growth and productivity. Acclimatization to drought stress is mediated via subtle alteration in gene expression which proceeds to changes in composition of plant transcriptome, proteome and metablome.

In many parts of our globe, sufficient water is not always available for cultivation due to poor irrigation facilities. This alarming situation makes drought one of the most adverse environmental factors. Promising way to make sure future food needs of the rising populations should involve an efficient use of water by the development crop varieties which need less amount of water and more tolerance to drought.

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