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sur le développement des zones arides et semi-arides

Titre : Screening of rice Oryza sativa L mapping population for submergence and drought tolerant traits

Auteur : Mishra, Vishwash Kumar

Université de soutenance : Acharya Narendra Deva University of Agriculture and Technology, Ayodhya

Grade : Doctor of Philosophy (PhD) 2020

Résumé
An experiment was conducted with four rice genotypes / lines, NDR-9830102, NDR-97, Swarna Sub1, Swarna Sub1 and rice lines NDR70-1, NDR-70-2, NDR-70-3 and NDR-70-4 to evaluate the submergence and drought related traits in pot and field condition. Thirty five days old rice seedlings were exposed to Submergence treatment. Plant height, days to 50% flowering, days to maturity was recorded at the end of submergence treatment. The rice genotypes / lines NDR-9830102 showed high increase in plant height while Swarna Sub1 and some plants of mapping population showed less increase in plant height. High catalase and peroxidase activity was recorded in Swarna sub1 and some mapping population plants and less in Nagina 22. Nagina 22 failed survives after 20 days of desumergence. Survived plant exposed to 15 days drought stress at reproductive stage. Swarna Sub1, NDR 9830102 and rice lines showed less percent reduction in days to 50% flowering and maturity over others. Relative water content (RWC), leaf rolling and proline content were recorded at the end of drought treatment. High proline content, RWC and less leaf rolling were recorded in NDR-9830102 and some plant of mapping population under drought stress environment. The NDR9830102 and rice lines both submergence and drought resistance traits. Parental polymorphism of rice genotypes were done by 17 SSR primers tested for validation of submergence and drought related traits in parent Swarna Sub1, Nagina-22 and lines. Out of 3 markers, only RM 201, RM 215 and 219 confirmed the drought tolerant traits.RM 219 amplified in both submergence and drought tolerance rice plants. By the phenotyping of rice lines under stress condition it was observed that only four plants showed the submergence tolerance at vegetative stage and drought tolerance at reproductive stage.

Mots clés : Biotechnology Genetics and Heredity Life Sciences Molecular Biology and Genetics

Présentation et version intégrale (Shodhganga)