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Chaudhary Charan Singh University (2014)

Studies on Inducet mutations in cowpea vigna ungulculta L walp

Devmani

Titre : Studies on Inducet mutations in cowpea vigna ungulculta L walp

Auteur : Devmani

Université de soutenance : Chaudhary Charan Singh University

Grade : Doctor of Philosophy (PhD) 2014

Sommaire partiel
The present investigation was undertaken to standardize the mutation breeding methodology with a view to explore the possibility of rapid varietal development over hybridization. Therefore, an attempt was made to develop an effective screening technique to identify promising progenies in the mutagenized population of Cowpea [Vigna unguiculata (L.) Walp]. Efforts were mainly directed to evolve a system of selection for polygenic variability in earlier generation M2 which would enhance the efficiency of breeding for polygenic characters through induced mutagenesis. The immediate effect of mutagenic treatments on germination, plant survival and pollen sterility was studied in M1 generation. In general, all the mutagenic treatments caused considerable damage to the genetic material. Dose-dependent increase in biological damage was recorded with all the three mutagens used. Among the three mutagens treated, gamma rays caused maximum biological damage, followed by EMS and combined in M1 generation. In order to identify plants with maximum mutagenic damage, the M1 plants were classified in three classes i.e. germination, plant survival and pollen sterility parameters so that the highly damaged plants can be identified early, as they are expected to yield mutations with higher frequency in the succeeding generations. The macro mutational studies in M2 generation revealed many interesting features. There was a clear parallelism between the frequency of chlorophyll and morphological mutations depending on the mutagens and doses. However, chlorophyll mutations appeared with higher frequency than morphological mutations. The frequency of both chlorophyll and morphological mutations was almost linearly dose dependent in case of gamma-rays, whereas, in case of chemicals, the medium dose gave highest frequency of both chlorophyll and morphological mutations. Among the mutagens used EMS (%) showed maximum effectiveness in including macro mutations, followed by combined and gamma rays

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