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Dayalbagh Educational Institute (2016)

Molecular Characterization of genes involved in Heat Stress Response in goats

Sharma, Richi Nagayach

Titre : Molecular Characterization of genes involved in Heat Stress Response in goats

Auteur : Sharma, Richi Nagayach

Université de soutenance : Dayalbagh Educational Institute

Grade : Doctor of Philosophy (PhD) in Zoology 2016

Résumé
Barbari and Jamunapari goat breeds, abundantly found in the semi-arid zones, have an ability to produce high milk and meat. They have thus been used as model breeds in the present study to monitor the severe effects of thermal stresses. For this work samples have been collected during summer, winter and comfort seasons and have been used subsequently analysed. Hsp70 gene (HSPA1A) has been detected, characterized and its sequence variation analysis has been done. The different physiological parameters, that is, rectal temperature (RT), respiratory rate (RR) and heart rate (HR) of these two goat breeds, Barbari and Jamunapari during summer, winter and comfort season have been recorded. Detection and expression profile of hsp70 gene (HSPA4) has been done in different tissues that is liver, heart, spleen and kidney. The severity of stress has been measured by temperature-humidity index calculation (THI) from goat breeds in heat, comfort as well as cold stress conditions. Besides these the isolation, detection, characterization and relative expression profile of specific hsp70 protein extracted from four different tissues under thermal stress conditions has also been done.

Présentation et version intégrale (Shodhganga)

Page publiée le 9 février 2021