Informations et ressources scientifiques
sur le développement des zones arides et semi-arides

Accueil du site → Doctorat → Inde → 2015 → Studies on the Enhancement of Soil Fertility by Microbes in degraded Land in Semi Arid Region of Secunderabad Telangana India

Amity University (2015)

Studies on the Enhancement of Soil Fertility by Microbes in degraded Land in Semi Arid Region of Secunderabad Telangana India

Singh Neha

Titre : Studies on the Enhancement of Soil Fertility by Microbes in degraded Land in Semi Arid Region of Secunderabad Telangana India

Auteur : Singh Neha

Université de soutenance : Amity University

Grade : Doctor of Philosophy (PhD) 2015

Résumé
Root colonising bacteria (rhizobacteria) that exert beneficial effects on plant development via direct or indirect mechanisms have been defined as plant growth promoting rhizobacteria (PGPR). In the present study, thirteen bacteria were isolated from the rhizospheric soil of Jatropha growing in degraded soil under semiarid conditions from southern India. Preliminary pot trials were carried out to investigate the interaction of these isolates with seeds of the test plant Jatropha curcas. Based on the preliminary results [Pot trials, Gram sand#8208 ;nature, Catalase test (qualitative test) and qualitative phosphate solubilisation], six strains were identified employing 16s rRNA techniques. Furthermore, phosphate solubilisation activity, indole acetic acid, siderophore, exopolysaccharide, ammonia, catalase and HCN were characterised by in vitro experiments. Additionally, effect of these six rhizobacteria, viz. SJPB (Acinetobacter baumannii), SJPB-2a (Aeromonas hydrophila), SJPB-2b (Acinetobacter sp.), BJJ-4 (Pseudomonas alcaliphila), BJJ-5a (Klebsiella pneumoniae) and BJJ-7 (Pseudomonas brassicacearum), on Cyamopsis tetragonoloba under pot culture experimental conditions was investigated. In comparison to control treated seeds, highest germination percentage, seedling length vigour index (SLVI), relative root elongation ratio (RER), lateral root density index ; protein-, amino acids-, sugar-, and photosynthetic pigment-content ; and catalase-, peroxidise enzyme activity were observed with BJJ-5a, indicating the potential of harnessing the benefit of plant growth promoting rhizobacteria to improve the germination and biochemical content of Cyamopsis tetragonoloba. Moreover antagonistic activity of these six rhizobacteria was qualitatively assessed for cell wall degrading enzymes activity. In comparison to control, isolated rhizobacteria demonstrated varying potential for antifungal activity against the phytopathogen Fusarium oxysporium, suggesting the benefit of these PGPRs as potential bio-control agents. newline

Présentation et version intégrale (Shodhganga)

Page publiée le 26 février 2021