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Accueil du site → Doctorat → Inde → 2013 → Biochemical composition studies of some Halophytes belonging to chenopodiaceae family of Indian Thar desert growing in vivo and in vitro

Maharaja Ganga Singh University (2013)

Biochemical composition studies of some Halophytes belonging to chenopodiaceae family of Indian Thar desert growing in vivo and in vitro

Kaur, Preetpal

Titre : Biochemical composition studies of some Halophytes belonging to chenopodiaceae family of Indian Thar desert growing in vivo and in vitro

Auteur : Kaur, Preetpal

Université de soutenance : Maharaja Ganga Singh University

Grade : Doctor of Philosophy (PhD) 2013

Préface
The present work deals with the study "Biochemical composition studies of some halophytes belonging to chenopodiaceae family of Indian Thar desert growing in vivo and in vitro’’ which has been divided into ten chapters. The review of Hterature and introduction of all chapters has been given separately in the beginning, whereas general discussion and summary are placed at the end of the chapters. Table and figures accompany the relevant chapters. Pertinent bibliography has been cited in the last. The plant material of Haloxylon recurvum, Haloxylon salicornicum and Salsola baryosma was collected from Central Arid Zone Research Institute (CAZRI), Bikaner. All the plant parts were separated, dried in shade, powdered and used for ftirther investigation. Complete description of the test plants have been pertaining to its systematic position, plant description, cultivation, medicinal properties and uses (Chapter I). Amino acids have been analyzed qualitatively and quantitatively from various plant parts viz. root, shoot and flower of all the three plant species (Chapter II). Plant parts viz. root, shoot and flower have been analyzed quantitatively for mineral content estimation such as dry matter, nitrogen, crude protein, ether extract, crude fiber, total ash, nitrogen free extract, total carbohydrates, and mineral contents like calcium and phosphorus (Chapter III). Unorganized callus cultures were established on suitable culture medium, using fresh, aseptically grown seedlings H. recurvum) and nodal segments H. salicornicum and S. baryosma) as explants. The cultures were harvested at their maximum Growth Indices of eight weeks, dried, powered and used for biochemical analysis (Chapter IV). The free endogenous ascorbic acid has been estimated in all plant parts (root, shoot and flower) as well as in unorganized tissue at maximum growth index oiH. recurvum, H. salicornicum and S. baryosma (Chapter V). Crude extract of plant parts (root, shoot and flower) and callus tissues of H. recurvum, H. salicornicum and S. baryosma for flavonoids have been screened for their antimicrobial activity against Gram +ve bacteria, Gram -ve bacteria and fungal pathogen (Chapter VI). The flavonoid content have been identified, isolated and estimated quantitatively fi-om the plant parts (root, shoot and flower) and callus tissues oiH. recurvum, H. salicornicum and S. baryosma. (Chapter VII). The alkaloid content have been identified and isolated from plant parts (root, shoot and flower) and callus tissues of H. recurvum, H. salicornicum and S. baryosma. (Chapter VIII). Phytosterols have been identified, isolated and estimated quantitatively fi-om the plant parts (root, shoot and flower) and tissue culture at maximum growth index of H. recurvum, H. salicornicum and iS*. baryosma. (Chapter IX). Effect of incorporation of phenylalanine (precursor of flavonoid biosynthesis) in H. recurvum, H. salicornicum and S. baryosma at the different concentrations (25, 50, 75 and 100 mg PA/100 ml of medium) on growth and production of flavonoids in concerned plants was observed. (Chapter X).

Mots clés : Amino acids Chenopodiaceae family Halopkytes Life Sciences,Plant and Animal Science,Plant Sciences Tissue culture Xerophytes

Présentation et version intégrale (Shodhganga)

Page publiée le 18 février 2021