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Accueil du site → Master → Inde → 2015 → EVALUATION OF EXTREME AMBIENCE ASSOCIATED CHANGES IN BIOMARKERS OF OXIDATIVE STRESS IN INDIGENOUS PIG FROM ARID TRACTS

Rajasthan University of Veterinary and Animal Sciences, Bikaner (2015)

EVALUATION OF EXTREME AMBIENCE ASSOCIATED CHANGES IN BIOMARKERS OF OXIDATIVE STRESS IN INDIGENOUS PIG FROM ARID TRACTS

Ramdas Yadav

Titre : EVALUATION OF EXTREME AMBIENCE ASSOCIATED CHANGES IN BIOMARKERS OF OXIDATIVE STRESS IN INDIGENOUS PIG FROM ARID TRACTS

Auteur : Ramdas Yadav

Université de soutenance : Rajasthan University of Veterinary and Animal Sciences, Bikaner

Grade : Master of Veterinary Science 2015

Résumé
The present investigation was carried out to evaluate extreme ambience associated changes in biomarkers of oxidative stress in indigenous pigs from arid tracts. Blood samples were collected during moderate, hot and cold ambiences to determine antioxidant biomarkers and enzyme biomarkers of oxidative stress in the serum. In each ambience blood samples were collected and the animals were grouped according to age as 4-6 months, 6-8 months, 16 8-10 months, 10-12 months and 12-14 months. Antioxidant biomarkers of oxidative stress included vitamin A, vitamin C, vitamin E and glutathione. Enzyme biomarkers of oxidative stress included super oxide dismutase(SOD), glutathione reductase(GR), catalase (CAT), peroxidase (PO), gamma glutamyl transferase (GGT) and monoamine oxidase(MAO). The mean overall values in serum of indigenous pigs during moderate ambience (control) were 2.60±0.02 µmol L-1 for vitamin A ; 21.56±0.41 µmol L-1for vitamin C ; 7.00±0.01 µmol L-1 for vitamin E ; 8.00±0.01 µmol L-1 for glutathione ; 179.00±2.12 kU L-1for SOD ; 15.00±0.05 kUL-1for GR ; 75.00±0.50 kU L-1for CAT ; 65.00±0.40 kU L-1 for PO ; 55.00±0.46 U L-1 for GGT and154.00±1.33 UL-1 for MAO. The mean values of serum vitamin A, C, E and glutathione were significantly (p≤0.05) lower during hot and cold ambiences as compared to moderate overall mean value. The age effects were significant (p≤0.05) in moderate, extreme hot and cold ambiences. Age effect showed a significant (p≤0.05) increase in the mean values being highest in the animals of 12-14 months of age. The mean values of enzyme biomarkers were significantly (p≤0.05) higher during hot and cold ambiences as compared to moderate overall mean value. It was observed that increase in the values during hot ambience was greater than that of cold ambience. The age effects were significant (p≤0.05) in moderate, extreme hot and cold ambiences

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