Informations et ressources scientifiques
sur le développement des zones arides et semi-arides

Titre : Biochemical and biophysical characterization of drought tolerance in Wheat Triticum aestivum L

Auteur : Parihar Sunita

Université de soutenance : Mohan Lal Sukhadia University

Grade : Doctor of Philosophy (PhD) 2020

Résumé
Biochemical and physiological screening of contrasting genotypes/varieties will provide in depth information about the mechanisms of drought tolerance in tolerant genotypes/varieties. Therefore, in the present investigations, biochemical and biophysical characterization of drought tolerance were carried out in various genotypes/varieties of T. aestivum L. The proposed work-plan can be summarized as follows :-

Biochemical studies
• To study the drought-induced changes in ROS-scavenging enzymes i.e. guaiacol peroxidase, superoxide dismutase, catalase, and glutathione reductase in various wheat genotypes growing in pots under drought stress. • To study the changes in activities of other cellular enzymes [βfructofuranosidase (invertase)], nitrate reductase, acid phosphatase, and α-amylase) in various wheat genotypes growing in pots under drought stress. • To study the drought-induced changes in cellular metabolites (proline, reducing sugar, and starch) in various wheat genotypes growing in pots.

Biophysical studies
• To analyse the response of drought on polyphasic chlorophyll a fluorescence OJIP kinetics (F50μs, F100μs, F300μs, FM) of T. aestivum growing in pots under drought stress. • To analyze the specific energy fluxes (per QA-reducing PSII reaction center—RC) such as ABS/RC (absorption flux per RC), TR/RC (trapped energy flux per RC), ET/RC (electron transport flux per RC), and DI/RC (dissipated energy flux per RC) in various genotypes of wheat growing in pots under drought condition. • To determine the phenomenological energy fluxes (per excited cross section – CS) such as ABS/CS (absorption flux per cross section), TR/CS (trapped energy flux per cross section), ET/CS (electron transport flux per cross section), and DI/CS (dissipated energy flux per cross section) in various genotypes of wheat growing under drought stress. • To evaluate the density of active and inactive PSII reaction centers (RC/CS) in various wheat genotypes growing under drought stress. • To analyze the yields or flux ratios such as ϕPo (maximum quantum yield of primary photochemistry), ϕEo (quantum yield of electron transport), and ϕDo (quantum yield of energy dissipation) in various genotypes of wheat growing under drought stress. • To study the photosynthetic performance indexes i.e. PIABS (performance index on absorption basis) and PICS (performance index on cross section basis) in various genotypes of wheat plants growing under drought stress

Présentation et version intégrale (Shodhganga)