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Accueil du site → Master → Inde → 2012 → MOLECULAR CHARACTERIZATION AND ADVANCED SCREENING FOR RESISTANCE IN GROUNDNUT FOR PEANUT BUD NECROSIS VIRUS

University of Agricultural Sciences, Raichur (2012)

MOLECULAR CHARACTERIZATION AND ADVANCED SCREENING FOR RESISTANCE IN GROUNDNUT FOR PEANUT BUD NECROSIS VIRUS

MUTTANNA REVADI

Titre : MOLECULAR CHARACTERIZATION AND ADVANCED SCREENING FOR RESISTANCE IN GROUNDNUT FOR PEANUT BUD NECROSIS VIRUS

Auteur : MUTTANNA REVADI

Université de soutenance : University of Agricultural Sciences, Raichur

Grade : Master of Science (MS) 2012

Résumé
Peanut bud necrosis disease is an economically important and serious disease causing considerable yield loss in groundnut crop. The disease is characterized by production of primary and secondary symptoms such as minute chlorotic and necrotic spots on quadrifoliate leaves, necrosis of terminal bud, stunting, bushy appearance, auxiliary shoot proliferation and malformation of leaflets. The virus causing peanut bud necrosis disease in different samples from eleven places of North East Karnataka were confirmed as peanut bud necrosis virus through RT-PCR. The molecular characterization of Raichur peanut bud necrosis virus isolate revealed that coat protein gene had codons for 272 amino acids and it has 100 per cent sequence homology with earlier reported isolates of peanut bud necrosis virus. The rate of epidemic development was highly influenced by the resistance levels of the host plant and conduciveness of the environmental condition. The rate of epidemic development and termination of diseases epidemic was independent of the disease pressure and it was at 85 days after sowing during kharif and rabi/summer. Among twenty promising interspecific derivatives of groundnut entries screened under epiphytotic field and glasshouse condition, seven entries viz., CS-43, CS-54, CS-55, CS-73, CS-77, CS-82 and CS-104 recorded resistant reaction for peanut bud necrosis disease along with good yield and desirable pod features. Diversity in twenty resistant groundnut genotypes revealed that all 23 primers tested over 21 groundnut accessions produced clear polymorphism. The similarity co-efficient ranged from 0.29 to 0.99 indicating a good level of divergence. Among all the genotypes, the highest similarity of 100 per cent was found between CS-77 and CS-82 and lowest of 75 per cent between CS-94 and CS-92 along with CS-86.

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