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University of Agricultural Sciences, Dharwad (2013)

Identifying drought tolerant cotton (gossypium hirsutum l.) genotypes by physiological and molecular approaches

Babu A. G.

Titre : Identifying drought tolerant cotton (gossypium hirsutum l.) genotypes by physiological and molecular approaches

Auteur : Babu A. G.

Université de soutenance : University of Agricultural Sciences, Dharwad

Grade : Doctor of Philosophy (PhD) 2013

Résumé
A field experiment was conducted during 2011-12 for the evaluation drought tolerance using thirty Gossypium, hirsutum L. genotypes in RBD design at ARS Dharwad and ARS Annigeri. Ten best (CPD-750, Sahana, ARB-9701, CNH-120MB, GIHV-218, BS- 279, RAH-101, GJHV-477, F-2228 and KH-155) and two least (L-761 and LH-2076) performed genotypes were selected from these experiments based on drought tolerant physiological traits viz. SLW, water potential, SPAD values, gas exchange parameters and yield. Simultaneously 600 cotton germplasms lines were also evaluated for drought tolerance by physiological traits at ARS Dharwad. Based on the SPAD, SLW and gas exchange parameters 24 germplasm lines were showed significantly on par for the most of the physiological traits. During 2012-13, the selected twelve genotypes were evaluated for performance under different concentrations of PEG-6000 osmotic solutions at germination stage by slanting glass plate technique. The germination %, shoot length, seedling vigour index and shoot vigour index decreased as the PEG 6000 concentration increase from 0% to 27% in all genotypes, while the root length and root : shoot ratio increased. These genotypes were further evaluated at rain out shelter for drought tolerance by morphological, physiological, biophysical, biochemical, root architecture (length, volume and lateral roots) yield, yield components and fibre properties. The genotypes viz. CPD-750, Sahana, ARB-9701, CNH- 120MB, GIHV-218, and BS-279 were found relatively drought tolerant. Further these twelve genotypes were studied for genetic diversity study using 79 cotton specific RAPD molecular markers. There was 79.56 per cent genetic polymorphism/diversity. The primers OPI 4 and OLIGO 652 amplified 800 bp and 400 bp respectively were specific to relatively drought tolerant genotypes. The dendrogram revealed that the primers were able to group the genotypes in to three major clusters. From the overall present investigation, the genotypes viz. CPD-750, Sahana, ARB-9701, CNH-120MB, GIHV-218, BS-279 and RAH-101 were identified as drought tolerant lines.

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