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Accueil du site → Doctorat → Allemagne → 2020 → Mapping and identifying genes for drought tolerance in barley (Hordeum vulgare L.)

Martin-Luther-Universität Halle-Wittenberg (2020)

Mapping and identifying genes for drought tolerance in barley (Hordeum vulgare L.)

Tarawneh, Rasha A

Titre : Mapping and identifying genes for drought tolerance in barley (Hordeum vulgare L.)

Auteur : Tarawneh, Rasha A

Université de soutenance : Martin-Luther-Universität Halle-Wittenberg

Grade : Doktorgrades der Agrarwissenschaften (Dr. agr.) 2020

Résumé
Drought is one of the most important limiting factors for crop production, affecting food security worldwide. The main objective of the present study was to identify marker trait associations (MTAs) and putative candidate genes related to different, morphological, agronomical and seed quality traits under pre- and post-anthesis drought stress by performing genome-wide association study (GWAS) using a diverse panel (EcoSeed) of 184 spring barley accessions. Pre-anthesis drought stress experiment was done based on high-throughput phenotyping using the automated phenotyping and imaging platform LemnaTec-Scanalyzer 3D in a greenhouse. Post-anthesis drought stress experiment was conducted in fields in two growing seasons 2016 and 2017, where drought stress was simulated by spraying 1% KI ten days after anthesis. Drought had impact on barley growth and development and finally the yield. GWAS analyses elucidated the genetic background of drought stress tolerance in barley revealed by a high number of MTAs on different chromosomes. Candidate genes were identified for associated traits, particularly genes involved in defense and drought tolerance. Results confirm that the SNP-gene based haplotypes could be considered as a primary screening manner to validate the SNPs within the candidate genes and to discover favorable alleles.

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Page publiée le 30 mai 2021