Informations et ressources scientifiques
sur le développement des zones arides et semi-arides

Accueil du site → Master → Etats Unis → 2019 → Assessing Rotifer Diversity in Desert Wetlands Through Sediment Rehydration and Amplicon Sequencing

University of Texas at El Paso (2019)

Assessing Rotifer Diversity in Desert Wetlands Through Sediment Rehydration and Amplicon Sequencing

Samaniego Sergio David

Titre : Assessing Rotifer Diversity in Desert Wetlands Through Sediment Rehydration and Amplicon Sequencing

Auteur : Samaniego Sergio David

Université de soutenance : University of Texas at El Paso

Grade : Master of Science (MS) 2019

Résumé
Species living in temporary wetlands can be threatened due to the instability and fluctuation of rising temperatures and evaporation caused by global climate change. In addition, regions characterized by dry climates, where most temporary wetlands exist, are expected to have a stronger response to climate change than other ecosystems. While most organisms are not able to respond to rapid changes in their environments, others like freshwater invertebrates have shown great resiliency to short- and long-term ecological disturbances. Rotifers for example, inhabit temporary waters that remain desiccated for indefinite periods of time making assessment of rotifer biodiversity challenging. Fortunately, many microinvertebrates have the ability to produce resting stages that persist in surface sediments when conditions become unfavorable. Their ability to persist through resting stages is important since the structure of these communities contribute to healthy and sustainable ecosystems. Here, active rotifer communities were compared with egg banks found in surface sediments from 12 temporary desert wetlands. Dormant communities were analyzed through rehydration experiments and amplicon sequencing. It was hypothesized that more permanent ponds would have higher microinvertebrate species richness than those that are more ephemeral due to habitat stability. Rehydration of sediments from 12 sites resulted in 41 rotifer species emerging from diapause, with richness ranging from 1-16. Unique taxa not present in active species lists were found in 8 out of the 12 sites. Species richness was highest in larger, Australian sites with lowest richness occurring in small rock pools from the United States.

Présentation

Page publiée le 27 mai 2021