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University of Belgrade (2013)

Composition, antimicrobial and antioxidant activity of the essential oils of Thumus algeriensis, Thymus capitatus, Satureja thymbra and Salvia fruticosa from Libya

Giweli, Abdulhmid Ahmed

Titre : Composition, antimicrobial and antioxidant activity of the essential oils of Thumus algeriensis, Thymus capitatus, Satureja thymbra and Salvia fruticosa from Libya

Састав, анимикробна и антиоксидативна активност етарских уља Thymus algeriensis, Th. capitatus, Salvia fruticosa и Satureja thymbra из Либије

Auteur : Giweli, Abdulhmid Ahmed

Université de soutenance  : University of Belgrade,

Grade : Doctoral dissertation 2013

Résumé
The essentiasl oils composition of some essential o ils from Lamiaceae family wild growing in Libya ( Thymus capitatus, Thymus algeriensis, Satureja thym bra and Salvia furticosa ) have been analyzed using GC and GC-MS. The oils o f Thymus capitatus, Th. algeriensis and Salvia fruticosa were characterised with domination of oxygenated monoterepenes with percentage 87.60%, 54 .67% and 64.89 % respectively. Monoterpene hydrocarbons was the maj or group of the compounds of the oil of S . thymbra represented 58.57% of the total oil. The main compounds of Th. capitatus essential oil were carvacrol (68.19%) followed by thymol (12.29%), while Th. algeriensis essential oil was characterized by thymol (38.50%) as the major component. The oil of S . thymbra was demonstrated by γ - terpinene (39.23%), thymol (25.16%) and p -cymene (7.17%) as the major constituents. S . fruticosa oil was characterized by contained 1,8-cineole (49 .34%) and camphor (7.53%) as the main compounds. The oils wer e also, screened for antioxidant activity by DPPH assay, and compared wi th their main compounds. The highest antioxidant activity showed S. thymbra oil with IC 50 = 0.0967 mg/ml of solution, followed by Th. capitatus (IC 50 = 0.119 mg/ml, Th. algeriensis with the IC 50 of 0.299 mg/ml and the lowest activity was found fo r S. fruticosa (IC 50 = 15.53 mg/ml of solution), comparing with 0.403 and 0.105 mg/ml for thymol and carvacrol, and 0.0717 mg/ml of solution for BHA. Furthermore, anti microbial activity was tested using microdilution method against eight bacteria a nd eight fungal species. Gram- negative from bacteria : Escherichia coli (ATCC 35210), Pseudomonas aeruginosa (ATCC 27853), Salmonella typhimurium (ATCC 13311), Proteus mirabilis (human isolate) and from Gram-positive bacteria : Listeria monocytogenes (NCTC 7973), Bacillus cereus (clinical isolate), Micrococcus flavus (ATCC 10240), and Staphylococcus aureus (ATCC 6538) were used...

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