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Accueil du site → Doctorat → Chine → 2020 → Fungal Biodiversity of Sand-fixed Plant Rhizosphere Soil and Antifungal Mechanisms of Some Trichoderma Species in Tengger Desert

Gansu Agricultural University (2020)

Fungal Biodiversity of Sand-fixed Plant Rhizosphere Soil and Antifungal Mechanisms of Some Trichoderma Species in Tengger Desert

郭成瑾;

Titre : Fungal Biodiversity of Sand-fixed Plant Rhizosphere Soil and Antifungal Mechanisms of Some Trichoderma Species in Tengger Desert

Auteur : 郭成瑾;

Grade : Doctoral Dissertation 2020

Université : Gansu Agricultural University

Résumé partiel
Rhizosphere soil fungi of sand-fixed plants is a kind of very important fungal resources,which plays an important role in the formation of sand soil structure,the balance of microflora,the utilization of beneficial microbial resources and promoting plant growth etc.However,there are few studies on community structure and diversity of rhizosphere soil fungi in sand-fixed plants,and the research of utilizing Trichoderma spp.resource in desert environment is also less.Therefore,this study aimed at the weak situation of research and utilization of rhizosphere soil fungi in sand-fixed plants,combining the random survey and the fixed point research method,using taxonomy of fungi and high-throughput sequencing technology to study community structure and diversity of rhizosphere soil fungi in sand-fixed plants in Tengger Desert in Ningxia ;By isolating,screening,identifying,analyzing biological characters of Trichoderma spp.of rhizosphere soil in sand-fixed plants,unveiling the antagonism mechanisms of Trichoderma spp.against Rhizoctonia solani ;evaluating the biological control of Trichoderma spp.against potato black scurf(R.solani),and on the micro-ecosystem of rhizosphere soil.The purpose of this research is to reveal the change of community structure and diversity of rhizosphere soil fungi in sand-fixed plants,to clarify the antifungal mechanisms of Trichoderma spp.against potato black scurf.The results provides a basic theory which has a significant meaning in desert ecological management and remediation of western China,and it’s also contribute to developing and applying beneficial microbial resources in extreme habitats.1.The cultivable fungi community structure and distribution characteristics of rhizosphere soil in sand-fixed plants.By combining the random survey and the fixed point research method,the structure and distribution characteristics of cultivable fungal community of rhizosphere soil in sand-fixed plants under different vegetation,time and habitat were studied.The results showed that the quantity of cultivable fungi was 6.38×103232.28×103 CFU·g-1 and the fungal species was 29 genera from rhizosphere soil in 52 species of sand-fixed plants ;the quantity and species of fungi in Peganum harmala were the highest,while those in Kalidium foliatum were the lowest.3176 fungal isolates belonging to 25 genera were isolated from rhizosphere soil in sand-fixed plants,of which species of Penicillium,Fusarium and Aspergillus were ubiquitous ;the quantity and species of soil fungi reached a peak in summer ;from 2013 to 2016,the quantity of soil fungi increased by 15.86%,while the species of soil fungi remained unchanged ;the quantity of soil fungi in steppe region was the highest,while that in desert region was the lowest ;the species of soil fungi in desert region was the highest,while that in closing protective region was the lowest ;Trichoderma was the dominant genus in desert region,Penicillium was the dominant genus in semi-desert region and steppe region,and Penicillium and Monilia were the dominant genus in closing protective region.2.The diversity of cultivable fungal community of rhizosphere soil in sand-fixed plantsBased on the survey data of quantity and species of soil fungi,the diversity,ecological distribution and soil character correlation of cultivable fungi community of rhizosphere soil in sand-fixed plants were studied.The result showed that summer had the highest fungal diversity index and richness,and the lowest Pielou eveness,while autumn had the lowest fungal diversity index and richness,and the highest Pielou eveness ;fungal diversity index,Pielou eveness and richness in 2013 were higher than those in 2016 ;fungal diversity index,

Mots clés : sand-fixed plant; fungal diversity; Tengger Desert; Trichoderma spp.; antagonistic mechanism; high-throughput sequencing; potato black scurf;

Présentation (CNKI)

Page publiée le 15 juin 2021