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Zhejiang University (2020)

Molecular Physiology and Evolution of Ion Transport and Drought Tolerance in Stomata of Wild Barley


Titre : Molecular Physiology and Evolution of Ion Transport and Drought Tolerance in Stomata of Wild Barley

Auteur : 陈光;

Grade : Doctoral Dissertation 2020

Université : Zhejiang University

Résumé partiel
Drought is one of the major abiotic stresses affecting sustainable agricultural development worldwide.Understanding the physiological and molecular mechanisms of drought tolerance is essential for breeding the drought-tolerant crops to cope with global climate change.Stomata act as the gate of gas and water exchange between the interior of leaf and the atmosphere,which tightly regulate photosynthesis and water use efficiency.Wild barley(Hordeum spontaneum)is highly tolerance to virous hostile environmental conditions and owns a wide genetic diversity,which make it an ideal model for revealing the crop abiotic stresses.In the present study,a series of comprehensive physiological,comparative genomic,transcriptomic,molecular,cell biological,and evolutionary biological dataset demonstrated the distinct difference among wild barley genotypes and populations,their evolutionary adaption to drought,and the functional diversity of key stomatal regulating anion channels.The results will provide useful information for the breeding of resilient barley for sustainable agriculture that is threatened by the changing global warming climate with higher temperature and more frequent drought events.The key findings of this research are:1.Evolutionary conservation of ABA signaling for stomatal closure in land plantsThe origin and evolution of mechanism of abscisic acid(ABA)-driven stomatal regulation in land plants is still under debate.36 species including algae and evolutionary representative land plants were selected in this study,and comparative genetic methods were used to analyze 23families of proteins related to ABA signaling pathway.The aquatic ferns Azolla filiculoides and Salvinia cucullata have representatives of 23 families of proteins orthologous to those of Arabidopsis(Arabidopsis thaliana)and all other land plant species studied.Moreover,comparative transcriptomic analysis has identified a suite of ABA responsive genes that differentially expressed in a terrestrial fern species,Polystichum proliferum,which fit the general ABA signaling pathway constructed from Arabidopsis and barley.Furthermore,stomatal assays have shown the primary evidence for ABA-induced closure of stomata in two terrestrial fern species P.proliferum and Nephrolepis exaltata.Phylogenetic analysis of the key ABA signaling proteins such as slow anion channels(SLACs)and open stomata1(OST1s)indicates an evolutionarily conserved stomatal response to ABA.In summary,new molecular and physiological evidences was found for the presence of active stomatal control in ferns.

Mots clés : Hordeum spontaneum; Stomata; Drought tolerance; Molecular evolution; ABA signaling pathway; ion transport; RNA-Seq; Whole genome resequencing; Slow anion channels(SLAC1/SLAH);

Présentation (CNKI)

Page publiée le 15 juin 2021