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Accueil du site → Doctorat → Chine → 2020 → Study on the Response Mechanisms of Foxtail Millet(Setaria Italica L.) Seedlings to Drought Stress Based on Transcriptome and Proteomics

Northwest A&F University (2020)

Study on the Response Mechanisms of Foxtail Millet(Setaria Italica L.) Seedlings to Drought Stress Based on Transcriptome and Proteomics

徐冰沁;

Titre : Study on the Response Mechanisms of Foxtail Millet(Setaria Italica L.) Seedlings to Drought Stress Based on Transcriptome and Proteomics

Auteur : 徐冰沁;

Grade : Doctoral Dissertation 2020

Université : Northwest A&F University

Résumé partiel
Foxtail millet(Setaria italica L.),belonging to the C4crop,has the characteristics of drought resistance,soil barren tolerance,and strong adaptability in wide area.It is one of important and special agricultural crops in China,that maintains agricultural economic stability in arid and semi-arid areas.As an abiotic stress,drought seriously inhibits its growth and development,and restricts the improvement of yield.Therefore,it is of great significance to carry out molecular biology research on drought resistance of foxtail millet,to reveal the genetic background and molecular regulation mechanism of foxtail millet drought resistance,and to improve the drought resistance of foxtail millet varieties.In this study,two foxtail millet cultivars,the drought-resistant cultivar"Damaomao"(DM)and the drought-sensitive cultivar"Hongniangu"(HN),were used as the experimental materials under the PEG6000-simulated drought stress at the seedling stage(three-leaf stage).Physiological and molecular regulation mechanism of foxtail millet response to drought stress were studied by analyzing the physiological and biochemical parameters,and through the analysis of the expression patterns of the transcriptome and proteome between the treatments and the control.The main research results obtained are as follows :(1)When subjected to drought stress,the two foxtail millet varieties showed a decrease in leaf width,leaves turning yellow and wilted,above-ground biomass decreased significantly,root-shoot ratio increased,and the net photosynthetic rate(Pn)and stomatal conductance(Gs)were significantly inhibited.The content of chlorophyll a and b were both decreased significantly.The content of MDA,soluble sugar and proline continued to increase with the time course of drought stress.The content of ABA and JA increased significantly within 24 hours.Compared with the drought-sensitive variety HM,the root-shoot ratio and proline content of the drought-tolerant variety DM increased more,and the MDA accumulation level,RWC and chlorophyll content decreased less,indicating that the metabolic activity of the DM could be maintained at a certain level,and its drought tolerance is stronger than that of HN,which is consistent with the field observation data.

Mots clés : Foxtail millet; drought stress; physiological response; transcriptome; proteome; differential response;

Présentation (CNKI)

Page publiée le 15 juin 2021