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Brigham Young University (2009)

Physiological Assessment of Chenopodium quinoa to Salt Stress

Morales, Arturo Jason

Titre : Physiological Assessment of Chenopodium quinoa to Salt Stress

Auteur : Morales, Arturo Jason

Université de soutenance : Brigham Young University

Grade : Master of Science 2009

Résumé
The physiological responses to salt stress were measured in Chenopodium quinoa. In a greenhouse experiment, salt water was applied to the quinoa varieties, Chipaya and KU-2, and to the model halophyte Thellungiella halophila to assess their relative responses to salt stress. Height and weight data from a seven-week time course demonstrated that both cultivars exhibited greater tolerance to salt than T. halophila. In a growth chamber experiment, three quinoa cultivars, Chipaya, Ollague, and CICA 17 were hydroponically grown and physiological responses were measured with four salt treatments. Tissues collected from the growth chamber treatments were used to obtain leaf succulence data, tissue ion concentrations, compatible solute concentrations, and RNA for real-time PCR. Stomatal conductance and fresh weight were measured to determine the degree of stress and recovery. The expression profiles of SOS1, NHX1, and TIP2, genes involved in salt stress, showed constitutive expression in root tissue and up-regulation in leaf tissue in response to salt stress. These data suggest that quinoa tolerates salt through a combination of exclusion and accumulation mechanisms.

Mots clé : quinoa, abiotic stress, halophila, salt, compatible solute, osmoprotectant, betaine, trigonelline, trehalose

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Page publiée le 13 septembre 2010, mise à jour le 10 novembre 2018