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Accueil du site → Doctorat → Chine → 2021 → Cloning and Characterization of GhYKT61 to Enhance Drought and Salt Tolerance in Plants

Chinese Academy of Agricultural Science (2021)

Cloning and Characterization of GhYKT61 to Enhance Drought and Salt Tolerance in Plants

Muhammad Askari

Titre : Cloning and Characterization of GhYKT61 to Enhance Drought and Salt Tolerance in Plants

Auteur : Muhammad Askari

Grade : Doctoral Dissertation 2021

Université : Chinese Academy of Agricultural Science

Résumé partiel
Abiotic stresses such as drought and salt severely affect the growth and development of plants,leading to a serious decline in crop yields.Soluble N-ethylmaleimide-sensitive factor attachment protein receptor(SNARE)is believed to be closely related to biological processes such as plant growth,development,cell homeostasis,and membrane fusion events in plants/crops.Many studies have shown that SNARE protein improves resistance to environmental stress by maintaining cell homeostasis.Drought and salt damage are the most important abiotic stresses in cotton production,which seriously affect its growth and development and yield formation.Although cotton is a pioneer crop tolerant to stress,cotton is mostly grown in arid and high-salt areas,and the intensified climate change in recent years has further aggravated the stress.Therefore,the use of agricultural biotechnology to improve the drought and salt tolerance of cotton and cultivate cotton varieties with high resistance to abiotic stress is of great significance for coping the climate change and ensuring stable and high yield of cotton.In this study,we used bioinformatics,biochemistry and molecular biology to clone the key R-SNARE gene Gh YKT61 from cotton in response to stress ;using VIGS to interfere Gh YKT61 expression by drought and salt tolerance revealed significantly reduced in cotton.On the contrary,Gh YKT61 overexpressing plants showed significantly improved stress tolerance.The results are described precisely below.RNA-Seq analysis showed that cotton Gh YKT61(Gh_DO8G278300)gene expression was very significantly induced by drought and high salt stress,and this induced expression pattern showed a relatively high consistency in the vegetative growth and reproductive growth stages,suggesting that the Gh YKT61 gene is important genes for cotton drought and high salt response.Based on this,we cloned Gh YKT61,full-length coding region of upland cotton were amplified and ligated in Blunt zero vector by reverse transcription and PCR.Gh YKT61 contains 6 exons and 5 introns and encodes a typical VAMP-like protein,which is widely present in plants,and there are a large number of homologous genes in cotton.Further q RT-PCR analysis found that Gh_DO8G278300 is the most significant Gh YKT61 family member induced under adverse stress conditions.We use virus-induced gene silencing(VIGS)technology to interfere to the Gh YKT61 gene in cotton.The expression analysis found that the Gh YKT61 gene expression interference in cotton lines by varying degrees of down-regulation.

Mots clés : Cotton ;GhYKT61;SNAREs ;Virus induced gene silencing ;Abiotic stress ;

Présentation (CNKI)

Page publiée le 16 novembre 2021