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Salt Tolerance Analysis and Creation of Transgenic Plants of GI Gene in Upland Cotton

Umar Akram

Titre : Salt Tolerance Analysis and Creation of Transgenic Plants of GI Gene in Upland Cotton

Auteur : Umar Akram

Grade : Doctoral Dissertation 2021

Université : Chinese Academy of Agricultural Science

Cotton is a natural fiber plant and the main raw material of textile industry.Cotton industry plays an important role in the national economy of the main cotton producing countries in the world and is one of the main economic crops.With the global warming,the contradiction between water shortage,energy shortage,food security and the growing population is becoming increasingly prominent especially in the field of agricultural production.In order to ensure food security,the excessive development and utilization of land resources lead to more and more serious soil salinization,which has brought huge losses to agricultural production.Cotton is a drought and saline-alkali tolerant crop,which can grow in the soil with more than 1%saline alkali content.It has now become the main direction of cotton industry development in China and other major cotton producing countries to plant salinity tolerant cotton in saline alkali soil and marginal soils for the sustainable production of important food crops.However,increasing soil salinity damage will also bring huge losses to cotton yield,which can lead to more than 90% reduction in cotton yield.It is of great significance for the development of cotton industry and food security to study the mechanism of cotton salt tolerance,excavate the related functional genes of cotton salt tolerance and cultivate new varieties of salt tolerant cotton.Previous reports have shown that gigantea(GI)is a plant specific protein,which controls the flowering time of plants.It is also a negative regulator of plant salt tolerance,affecting many genes involved in salt tolerance pathway.In this study,virus induced gene silencing(VIGS)was used to study the function of GI gene in upland cotton seedlings.At the same time,the GI homologous genes in upland cotton were knocked out by CRISPR/cas9 gene editing technology to create transgenic cotton materials with salt tolerance,and further study the function of this gene in the process of cotton growth and development.At the same time,all GI homologous genes in upland cotton were transformed into tobacco and cotton to create new transgenic materials.From this transgenic material,the activities of antioxidants SOD,cat and pod,and the expression patterns of salt hypersensitive protein(SOS)and sodium hydrogen transporter(NHXs)genes under salt stress were studied by ELISA and QRT PCR,respectively.In addition,in order to better understand the role of NHXs in the salt tolerance pathway of cotton,the whole genome analysis of all NHXs genes in cotton plants was carried out,the function of NHXs family was preliminarily predicted,and the expression patterns of main NHXs genes in root,stem and leaf tissues of cotton under Salt Stress were detected

Mots clés : Gigantea ;Genome Editing ;Salinity Tolerance ;NHXs Antiporters ;Protein-protein Interactions ;

Présentation (CNKI)

Page publiée le 15 novembre 2021