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Shandong Normal University (2021)

The Roles of NAC Transcription Factors in Responses to Drought Stress in Peanut

李鹏祥

Titre : The Roles of NAC Transcription Factors in Responses to Drought Stress in Peanut

Auteur : 李鹏祥

Grade : Master These 2021

Université : Shandong Normal University

Résumé partiel
Peanut(Arachis hypogaea)is the main oil crop and cash crop in the worldwide.Drought stress always causes the decline of yield and quality in peanut,and increases the risk of contamination by aflatoxins.With the intensification of global warming and the increasing in demand for peanut,the supply and demand of peanut will become increasingly prominent.Therefore,it is of great significance to reduce the adverse effects of drought on peanut production by improving the drought tolerance of peanut.NAC transcription factor(TF)is one of the most important transcription factors in plants,and it has multiple family members.The majorities of NAC proteins have the highly conserved NAC domain at N-terminal and the highly diversified transcriptional regulatory domain at C-terminal.They are widely involved in plant growth and development,and responding to biotic and abiotic stresses.So far,there is no systematic study on the NAC family in peanut.In this study,the NAC family genes in peanut were systematically studied by using a series of methods,including the phylogenetic analysis,chromosome location analysis,repeated event analysis,transcriptome expression data analysis,and qPCR verification.At the same time,the AhNAC14,AhNAC20 and AhNAC120 genes were selected for further analyses of expression profile and functional characteristics.These results will provide the foundation for the genetic improvement of peanut drought tolerance.The main results are as follows :(1)132 AhNACs were identified from the genome of cultivated peanuts and were divided into 8 subgroups(Ⅰ-Ⅷ)based on their phylogenetic relationship with Arabidopsis NAC proteins and the conserved motifs.These genes are unevenly distributed on all 20 chromosomes,including116 pairs of fragment duplication events and 1 pair of tandem duplication.Transcriptome analysis showed that many AhNAC genes responded to drought and abscisic acid(ABA)stress,especially most members of groups Ⅳ,ⅣI and Ⅷ,which were induced or inhibited in roots or leaves treated with PEG or ABA expression.In addition,the response patterns of 20 AhNAC genes to PEG and ABA treatment were verified by qRT-PCR.Most results of qRT-PCR analysis are consistent with the results of transcriptome analysis.(2)Cloning and sequence analysis of AhNAC14,AhNAC20 and AhNAC120 genes.The ORF of AhNAC14,AhNAC20 and AhNAC120 were cloned.Among them,the AhNAC14 is 751 bp in ORF size,which encodes 211 amino acids,and its theoretical molecular weight(MW)and isoelectric point(p I)are respectively 23.46 kDa and 9.45,and its g DNA contains 2 exons and 1intron ;The ORF size of AhNAC20 is 861 bp,the number of encoded amino acids is 286,and its theoretical MW and p I are 32.83 k Da and 8.48,respectively,and similar as AhNAC14,its g DNA also has 2 exons and 1 intron ;The ORF of AhNAC120 is 915 bp in size,which encodes 304 amino acids,and its g DNA contains 3 exons and 2 introns,and its theoretical MW and p I are 35.08 kDa and 6.42,respectively.It was predicted that these three proteins are hydrophilic proteins,and there are no possibility of transmembrane structures and signal peptides.

Mots clés : Peanut (Arachis hypogaea) ;NAC transcription factor family ;drought responding ;expression pattern analysis ;gene function ;

Présentation (CNKI)

Page publiée le 31 octobre 2021