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Accueil du site → Doctorat → Inde → 2019 → Isolation and characterization of thar desert actinomycetes producing useful secondary metabolites

Maharaja Ganga Singh University (2019)

Isolation and characterization of thar desert actinomycetes producing useful secondary metabolites

Begani, Jyotsna

Titre : Isolation and characterization of thar desert actinomycetes producing useful secondary metabolites

Auteur : Begani, Jyotsna

Université de soutenance : Maharaja Ganga Singh University

Grade : Doctor of Philosophy (PhD) 2019

Descriptif
The aim of the present research was to isolate desert actinomycetes from the four selected regions of Thar ecosystem in Rajasthan, India. The cultivable diversity of actinobacterial strains along with their environmental adaptation was investigated and their ability to produce antibacterial activity against various bacterial and fungal pathogenic strains was assessed. The research objectives addressed (Fig.1.4) in the present thesis are furnished below : • Isolation of thermophilic actinomycetes producing secondary metabolites from the desert ecosystem of Bikaner falling in an arid zone of the Thar in the Indian Territory. • Screening for antibiotic potential, phenotypic (morphology), physiological (biochemical) and genetic characterization (species-specific genomic primers) of the actinomycetes isolates from the above step. • Determining molecular phylogenetic relationship based on 16S rRNA gene sequences for the purpose of classification and taxonomic use. • Partial purification of secondary metabolites from actinomycetes by autobiography and preparatory Thin Layer Chromatography (TLC).

Mots clés : Actinobacteria desert soil, Life Sciences, Microbiology, Microbiology

Présentation et version intégrale (Shodhganga)

Page publiée le 28 octobre 2021