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Inner Mongolia University (2021)

Isolation and Identification of Three New Bacteria Including Sphingomonas from Inner Mongolia Grassland Soil

沈立新

Titre : Isolation and Identification of Three New Bacteria Including Sphingomonas from Inner Mongolia Grassland Soil

Auteur : 沈立新

Grade : Master 2021

Université : Inner Mongolia University

Résumé partiel
Soil microorganisms are rich in species and quantity resources.The most abundant microbial communities on earth exist,such as bacteria,archaea,fungi,viruses,protozoa and some micro animals.Soil microorganisms are found in almost all phylum,which has important research significance and broad application prospects.Through soil sampling and isolation and identification of soil microorganisms,we can systematically classify and identify the microorganisms that can be cultivated,enrich the types of soil microorganisms,and lay a foundation for the study of the role of soil microorganisms in biogeochemical cycle and ecology.In this paper,bacteria were isolated from three soil samples from Xiwuqi(meadow grassland),Maodeng pasture(typical grassland)and Saihantala(desert grassland)by traditional isolation method,and species classification was performed based on 16 S rRNA gene seqeuncing.In addition,two strains(XHJ-2T and MHJ-3QT)of Sphingomonas and a strain MHJ-10JT of Pseudomonas were identified as novel species using the multiphase taxonomy.The main results are as follows:1.A total of 136 strains of bacteria were isolated from three type grassland soil samples of Xiwuqi,Maodeng pasture and Saihantala.Baset on 16 S rRNA gene sequence,they belonged to four phyla including Firmicutes,Proteobacteria,Actinobacteria and Bacteroidetes,with 44 genera.The order of population complexity of isolated bacteria was Xiwuqi grassland soil sample > Maodeng pasture soil sample > Saihantala soil sample.2.Classification and identification of Sphingomonas sp.XHJ-2T and MHJ-3QT:Strains XHJ-2T and MHJ-3QT are novel species of genus Sphingomonas of Alphproteobacteria.XHJ-2T and MHJ-3QT cells were gram negative,short rod-shaped and without flagella free.Phylogenetic analysis based on 16 S rRNA gene sequences showed that strain XHJ-2T and MHJ-3QT was most closely related to Sphingomonas zeicaulis 541(97.09%)and Sphingomonas rosea PB196(97.31%),respectively.The genomic DNA G + C contents of XHJ-2T and MHJ-3QT were64.61% and 67.84%,respectively

Mots clés : grassland soil ;identification;Sphingomonas ;Pseudomonas;novel species ;

Présentation (CNKI)

Page publiée le 6 novembre 2021