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Tarim University (2021)

Diversity of Soil Bacterial Communities in the Eastern Margin of the Taklimakan Desert

张哲瑄

Titre : Diversity of Soil Bacterial Communities in the Eastern Margin of the Taklimakan Desert

Auteur : 张哲瑄

Grade : Master 2021

Université : Tarim University

Résumé partiel
Taklimakan desert,located in the hinterland of Tarim Basin in Xinjiang,is the second largest mobile desert in the world.Desert is not a barren land,which contains abundant and diverse microbial resources.The special geographical location,arid natural environment and frequent wind sand activities make the wind sand landform of Taklimakan desert complex and diverse,and also provide a special living environment for microorganisms.The interaction between halophytes such as Populus euphratica,Tamarix ramosissima and karelinia caspica and microbial community constitutes a unique rhizosphere microbial community structure.In this study,21 samples of different types of sandy soil were collected from 10 different sampling points in the eastern edge of Taklimakan desert in June 2019,including 10 wind-blow sand samples,6 Tamarix ramosissima ledeb rhizosphere soil samples and 5 Populus euphratica rhizosphere soil samples.The structure of microbial community in different habitats in the eastern edge of Taklimakan desert was revealed by culture-free technology,and the relationship between community structure and environmental was revealed by the analysis of environmental factors ;Actinomycetes were isolated from sandy soil samples by culture technique,and antagonistic strains of cotton verticillium wilt were screened ;and a new suspected actinomycete species was polyphasic separated identification.The specific research results include:1.In this study,the total DNA of Desert sandy soil samples was extracted by suction filtration combined with SDS-CTAB method,and purification the DNA by kit.The high concentration and complete genomic DNA was obtained.By sequencing 21 sand samples collected from the eastern edge of Taklimakan desert,1 412 200 valid sequences were obtained,the OTUs of samples from different habitats were 22 730 ;the number of OTUs in the east edge of Taklimakan desert was much higher than that of other samples(Populus euphratica rhizosphere soil and Tamarix ramosissima ledeb rhizosphere soil)in the same habitat ;the alpha diversity analysis of different sampling points in the eastern edge of Taklimakan Desert showed that,the OTUs of Washixia and Minfeng-Yutian were significantly higher than that of other samples(Populus euphratica rhizosphere soil and Tamarix ramosissima ledeb rhizosphere soil),Which meaned that,Washixia and Minfeng-Yutian have the highest microbial diversity and species abundance ;Venn diagram and heat-map showed that sandy soil samples accounted for more OTUs then other samples,while the samples collected near the Qiala reservoir had the strongest specificity ;By using UPGMA cluster analysis and NMDS analysis,We can found there was a certain similarity between microorganisms in each sampling area,and the same sample type could be clustered separately ;through OTU species annotation,304 genera belonging to 42 classes of 21 phyla were identified from the 21 samples collected in the eastern margin of Taklimakan Desert,The dominant phyla were Bacteroidetes,Proteobacteria,Actinobacteria and Firmicutes ;salinimicrobium have the highest relative abundance at the genus level,followed by Halomonas,sporophyta,Bacillus,Pontibacter,Aliifodinibius and Kocuria.Through the comparison of environmental factors,it is found that the differences of soil factors among the same sample types are extremely significant due to the changes of sampling areas.

Mots clés : Taklimakan desert ;Illumina MiSeq ;Diversity of bacteria ;Actinomycetes;Multi-phase classification ;

Présentation (CNKI)

Page publiée le 9 novembre 2021