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Accueil du site → Doctorat → Allemagne → 2021 → Adaptation assessment of a spring barley population to organic and conventional agro-ecosystems using genome sequencing approaches

Rheinischen Friedrich-Wilhelms-Universität Bonn (2021)

Adaptation assessment of a spring barley population to organic and conventional agro-ecosystems using genome sequencing approaches

Schneider, Michael

Titre : Adaptation assessment of a spring barley population to organic and conventional agro-ecosystems using genome sequencing approaches

Auteur : Schneider, Michael

Université de soutenance : Rheinischen Friedrich-Wilhelms-Universität Bonn

Grade : Doktor der Agrarwissenschaften (Dr. agr.) 2021

Résumé partiel
Over the past few decades, plant breeding has contributed significantly to increased crop yields in industrial agriculture. Besides, alternative, environmentally friendly farming approaches became more popular in the past years. However, questions arise which breeding goals are relevant to breed new varieties which are specially adapted for organic farming. The common practice of transferring conventionally adapted material into organic farming might lead to yield losses due to lacking adaptation of such varieties to organically farmed environments. So far, little is known about physiologically relevant characteristics for organically adapted varieties. To answer this question, a long-term selection experiment in a spring barley population was established in 1998. A twice backcrossed population with a cultivar as recurrent and a wild-type as donor parents was established and cropped in conventionally and organically managed farming environments for more than two decades. Mainly the farming environments with their adjustments in fertilization, crop rotation, and plant protection as well as weather impacts should have driven the selection of individual genotypes in these populations. Therefore, the artificial selection was reduced to a minimum so that only natural selection should lead to changes in the allele frequencies of the populations. In this thesis, complete populations for different generations and environments were genotyped entirely by applying a novel pool-based deep genotyping using a whole-genome resequencing approach. Implementing a haplotyping strategy makes it possible to dissect allele frequency variations on gene-level at low sequencing depth. Additionally, allele frequency variations between neighboring haplotypes have been used to calculate a consistent genetic map.

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Page publiée le 13 novembre 2021