Informations et ressources scientifiques
sur le développement des zones arides et semi-arides

Accueil du site → Projets de développement → Projets de recherche pour le Développement → 2021 → Chickpea Genetic Improvement for Drought and Heat Stress Resilient Grain Yield

United States Department of Agriculture (USDA) 2021

Chickpea Genetic Improvement for Drought and Heat Stress Resilient Grain Yield

Chickpea Drought Stress Genetic

United States Department of Agriculture (USDA) Agricultural Research Service (ARS)

Titre : Chickpea Genetic Improvement for Drought and Heat Stress Resilient Grain Yield

Identification : 3060-21650-001-035-

Pays : Etats Unis

Durée : Start Date : Aug 1, 2021 // End Date : Dec 31, 2022

Location : Sugarbeet and Potato Research Fargo ND

Objectifs
(1) Introduce genes into key U.S. chickpea cultivars by marker-assisted backcrossing for drought-tolerant yield from a cultivated chickpea ; (2) Introduce genes into key U.S. chickpea cultivars by marker-assisted backcrossing for high temperature pod setting from a recently found wild chickpea ; (3) Evaluate introgressed material from Objectives 1 and 2 under stress and non-stress conditions to identify top performing selections ; and (4) Develop functional molecular markers for further marker-assisted breeding into U.S. cultivars.

Approche
The U.S. elite cultivars ‘Nash’, ‘BillyBeans’ and ‘UC27’ were selected to represent the major market types (kabuli export, processing, canning respectively) and chickpea cultivation areas (Northern Plains, Pacific Northwest area, inland California respectively) and will be used as recurrent (elite) parents for introgression breeding. Due to the current lack of molecular markers for the U.S. elite material, F2 plants from these crosses have been selected with a morphological marker (purple flowers) that is loosely linked to the chromosome 4 drought tolerance Quantitative Trait Locus (QTL) of ICC4958 for ongoing backcrossing in summer 2021. For subsequent cycles of backcrossing, markers developed from Objective 4 will be used for marker assisted selection (MAS) to screen backcross individuals to select those harboring the ICC4958 chr4 haplotype as heterozygotes among BCnF1s and homozygotes in F2/3 progenies for phenotyping (Objective 3). For heat tolerance introgression, Kalkan F5s (identified in the 2017/8 field heat screen) will be grown with an elevated reproductive phase temperature (daytime high 35C and night low of 20C at/after flowering) in a greenhouse. The most heat tolerant (highest podding) individual F5 plants will be chosen for use in greenhouse crossing to the three U.S. elite cultivars. F1 hybrids obtained will be greenhouse grown, selfed, and F2 seed harvested. 300 greenhouse-grown F2 plants (100/elite parent) will be sampled for leaf tissue for use in heat tolerance QTL mapping/ marker development. For Objective 4, molecular markers for use in marker assisted backcrossing will be identified by using whole genome sequencing of the three US elite cultivars. The resulting Single Nucleotide Polymorphism (SNP) variant calls of US cultivars against ICC4958 will be filtered for SNPs at the chr4 QTL hotspot and 8-12 SNPs selected for conversion to KASP genotyping assays for use in marker assisted selection for the drought QTL. To identify markers for heat tolerance QTLs from the Kalkan donor, RADseq reduced representation sequencing will be done on the F2 introgression populations (3 x 100 F2s). To identify SNPs, RADseq data will be aligned to the whole genome data of the U.S. cultivars generated in Year 1. SNPs with data in >80% of each F2 population and intermediate frequencies will be selected for QTL mapping with phenotypic data. The top 1-2 QTLs will be prioritized for marker development using SNPs in QTL flanking regions for KASP marker development.

Présentation : USDA (ARS)

Page publiée le 1er décembre 2021