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Accueil du site → Doctorat → Brésil → Potencial hepatoprotetor, antineoplásico e genoprotetor da geoprópolis produzida por abelha jandaíra (melipona subnitida d.) no semiárido do rio grande do norte, brasil

Universidade Federal Rural do Semi-Árido (UFERSA) 2020

Potencial hepatoprotetor, antineoplásico e genoprotetor da geoprópolis produzida por abelha jandaíra (melipona subnitida d.) no semiárido do rio grande do norte, brasil

Paiva, Kaliane Alessandra Rodrigues de

Titre : Potencial hepatoprotetor, antineoplásico e genoprotetor da geoprópolis produzida por abelha jandaíra (melipona subnitida d.) no semiárido do rio grande do norte, brasil

Auteur : Paiva, Kaliane Alessandra Rodrigues de

Université de soutenance : Universidade Federal Rural do Semi-Árido (UFERSA)

Grade : Doutora no Curso de Pós-Graduação em Ciência Animal. 2020

Résumé
The objective was to evaluate the hepatoprotective potential against injuries induced by acetaminophen, the antineoplastic and genoprotective activity of the hydroethanolic extract of the geopropolis produced by Melipona subnitida D., in the semiarid region of Northeast Brazil. The chemical composition and antioxidant activity of the six samples (A1, A2, A3, A4, A5, A6) of geopropolis hydroethanolic extract were evaluated. The hepatoprotective potential was evaluated through biochemical analysis of liver function, macroscopic, histological and stereological evaluation of the livers in 04 experimental groups (G1- negative control, G2- 50 mg/kg of hydroethanolic extract from geopropolis, G3- 50 mg/kg of hydroethanolic extract of geopropolis and 600 mg/kg of acetaminophen, G4- 600 mg/kg of acetaminophen) consisting of 06 rodents of the species Rattus norvegicus Berkenhout, 1769, Wistar strain, with acute liver damage induced by acetaminophen. The antineoplastic potential was determined by evaluating the cytotoxic effect of the hydroethanolic extract of geopropolis in human hepatocellular carcinoma (HepG2) cell lines and fibroblasts from L929 mice. The genoprotective effect was analyzed in the L929 mouse fibroblast cell line. Samples of the geopropolis hydroethanolic extract showed 41 phenolic compounds, distributed between phenols and flavonoids. The presence of 4- demethyldeoxypodophyllotoxin 4-O-glucopyranoside is reported for the first time in a meliponid geopropolis. Samples A1, A2, A4, A5 and A6 showed moderate antioxidant activity expressed by IC50 values equal to 82.50, 122.20, 98.80, 76.30 and 144.0, respectively, correlated with high levels of total phenols and flavonoids ranging from 16.62 ± 3.91 to 28.46 ± 1.73 and 14.72 ± 3.75 to 32.22 ± 4.43, respectively. The results of the serum levels of the enzymes TGO and TGP of the rats, indicated that the hydroethanolic extract of the geopropolis of M. subnitida D. did not cause hepatotoxicity. In the macroscopic evaluation of the livers, in the G3 group it was possible to observe a pale brown color and slight hemorrhage, in the G4 the presence of hemorrhagic suffusions in the capsule, irregular contours and accentuation of the lobular pattern. In the histoarchitecture of the livers, in G3 it 8 was found foci of tumefied, vacuolized and necrotic hepatocytes, in G4 degeneration and diffuse necrosis of hepatocytes. In stereology, there was an increase in the percentage of hepatocytes and sinusoids, as well as a reduction in collagen in the G3 group compared to G4

Mots Clés  : Abelha sem ferrão Genoproteção Geoprópolis Lesão hepática Quimioterápico Stingless Bee. Genoprotection. Geopropolis. Hepatic lesions. Chemotherapy

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Page publiée le 16 décembre 2021