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University of Gezira (2020)

Extraction, Characterization and Determination of Physicochemical Properties of Elketan (Linum usitatissimum L.) Seed Oil

Mohammed, Twasul Abd Elmohssin Merghani

Titre : Extraction, Characterization and Determination of Physicochemical Properties of Elketan (Linum usitatissimum L.) Seed Oil

Auteur : Mohammed, Twasul Abd Elmohssin Merghani

Université de soutenance : University of Gezira

Grade : Master of Science (MS) in Chemistry 2020

Résumé
Vegetable oils have been used for centuries as source of energy and industrial applications. Flax is an annual, self-pollinate species, it provides raw materials for food, medicine and textiles. Flax seed is a rich source of different types of phenolics. This study aimed to determine its physicochemical properties such as fatty acid composition, antioxidant activity and to study flax seed oil refining. All the physicochemical properties were determined according to the American Oil Chemists Society (AOCS) official methods. Flax seed samples were collected from the Baraka shop -Wad Medani. Physicochemical properties of flax seed include moisture content, oil content, crude protein, and crude fiber. For flax seed these were 1.92%, 37.9%, 24%, 22% respectively. The proximate analysis of flax seed Oil (FSO) includes moisture content, free fatty acids, peroxide value, saponification value, unsaponifiable matter, refractive index, and color for FSO. These were 0.005%, 0.52%, 6.4 meq/kg, 146 mgKOH/g,0.031%,1.4818, Red : 2.7 Yellow : 70.0 respectively .Fatty acid composition was determined by GC-MS. The most abundant unsaturated fatty acids in the FSO were linolenic acid 50.01% in FSO followed by Linoleic acid 26.80% while the most abundant saturated fatty acids in the FSO was palmitic acid 11.9% followed by Stearic acid 9.35%. The antioxidant activity was determined by DPPH assay. The antioxidant activity of FSO extracted by cold pressing it was 20% while for the oil extracted by n-hexane in detectable. The oil has good physicochemical properties and is rich in unsaturated fatty acids. Cold pressing showed higher antioxidant activity than solvent extraction. Free fatty acids and color were determined for refined FSO as 0.051%, (Red 0.3 - yellow 4.1). It is recommended that further studies should be made to determine the minor components of the oil. Also the stability of the oil need to be studied because of its high contents of unsaturated free fatty acids.

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