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Al Neelian University (2021)

Phytochemical Investigation, Cytotoxic, Antioxidant and Antimicrobial Activity Assessment of Sudanese Fagonia indica Burm f. (Zygophyllaceae)

Muna Abdellatif Khalil Mohamed

Titre : Phytochemical Investigation, Cytotoxic, Antioxidant and Antimicrobial Activity Assessment of Sudanese Fagonia indica Burm f. (Zygophyllaceae)

Auteur Muna Abdellatif Khalil Mohamed

Université de soutenance : Al Neelian University

Grade : Doctor of Philosophy (PhD) in Chemistry 2021

Résumé partiel
Fagonia indica Burm f. ( Zygophyllaceae ) grows wild in the African and Asian deserts and is used for treatment of many diseases in traditional medicine of these countries. The present research is concerned with the species growing in Sudan with emphasis on its cytotoxic ; antioxidant and antimicrobial activities. The whole plant was extracted with 96% ethanol and the prepared extract was fractionated successively with petroleum ether 40–600C, followed by chloroform, ethyl acetate and n-butanol. The ethanolic extract and fractions were subjected to detailed phytochemical screening according to standard procedures and the results revealed the presence of alkaloids ; terpenoids ; saponins ; flavonoids and carbohydrates. Parallel to this , the ethanolic extract and fractions were tested for their cytotoxic ; antioxidant and antimicrobial activities. Mortality percent of Brine shrimp cytotoxicity of the 96% ethanolic extract and fractions showed that the lowest LD50 was exhibited by the aqueous extract (516.66 μg/ml), which supported its uses in traditional medicine as a safe herbal tea preparation. The petroleum ether ; chloroform ; ethyl acetate ; n-butanol and aqueous fractions showed better cytotoxic effect than the original ethanolic extract in the human tumor cell line ( Colon cell line ; Human hepatocellular carcinoma cell line ; Human caucasian breast adenocarcinoma and normal retina cell line). The best results were scored by chloroform fraction (97.4 % ) followed by ethyl acetate fraction (89.5%) and petroleum ether fraction (66.5%) at 100ppm concentrations. The antioxidant activities of the samples were assessed by the DPPH methods and the best results were scored by the 96% ethanolic extract (82±0.07) followed by ethyl acetate fraction (81±0.09) , n-butanol fraction (76±0.02) , aqueous fraction (75±0.01 ) , chloroform fraction (74±0.06 ) and finally pet. ether 40-600C fraction (39±0.09) . The antimicrobial activity assessment of the 96% ethanolic extract and fractions against Bacillus subtilis (B.s) ; Staphylococcus aureus (S.a) ; Escherichia coli (E.c) ; Pseudomonas aeruginosa (Ps.a ) and Candida albicans (C.a) revealed different results. Generally, the 96% ethanolic extract gave the best result against Ps.a.( 18-19mm MDIZ).

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Page publiée le 15 janvier 2022