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Accueil du site → Master → Arabie Saoudite → EVALUATING SULPHUR AND POTASSIUM INDUCED SALINITY TOLERANCE OF TOMATO (LYCOPERSICON ESCULENTUM MILL.) USING PHYSIOLOGICAL, BIOCHEMICAL AND MOLECULAR ANALYSES

King Abdulaziz University (2020)

EVALUATING SULPHUR AND POTASSIUM INDUCED SALINITY TOLERANCE OF TOMATO (LYCOPERSICON ESCULENTUM MILL.) USING PHYSIOLOGICAL, BIOCHEMICAL AND MOLECULAR ANALYSES

Al-Nefaie, Awidh Haifa

Titre : EVALUATING SULPHUR AND POTASSIUM INDUCED SALINITY TOLERANCE OF TOMATO (LYCOPERSICON ESCULENTUM MILL.) USING PHYSIOLOGICAL, BIOCHEMICAL AND MOLECULAR ANALYSES

Auteur : Al-Nefaie, Awidh Haifa

Université de soutenance : King Abdulaziz University

Grade : Master Thesis 2020

Résumé
Salinity is one of the major environmental stress, affecting agriculture crop productivity particularly in arid and semi-arid regions. The application of plant mineral fertilizer could be used as an effective strategy to alleviate salt stress. The present investigation was carried out to study the effect of Sulphur (S) in the form of MgSO4 (5mM), Potassium (K) in the form of KNO3 (10 mM) and the combination between them on salt tolerance, the study was conducted using salt-resistant and salt-sensitive tomato grown under NaCl (0, 50, and 250 mM). We investigated fresh and dry biomass of tomato shoot, chlorophyll pigment, lipid peroxidation, proline content, soluble protein content and sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) as an indication for plant resistance. All data were measured after two months of germination and 10 days of salt stress. The results indicated that all minerals treatment (S, K, S+K) significantly increased shoot fresh and dry weight of salt-sensitive genotype at 50 mM NaCl compared with control. Also, adding K and (S+K) showed significantly increased shoot length at 50 mM NaCl, and the highest number of leaves was when we added (S+K) at 50 mM NaCl. Salt-resistant K and (S+K) show a significant increase in Chlorophyll a b at 50 mM NaCl with control 50 mM NaCl. Whereas S and K increase Chlorophyll a+b at 250 mM NaCl. However, in salt-sensitive genotype only when adding S plant show increased in Chlorophyll a+b at 250 mM NaCl compared to the control 250 mM NaCl. All mineral treatments significantly increase proline and protein accumulation in plant shoot under saline conditions in each genotype. The mineral treatments alleviated the stress-induced lipid peroxidation.

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Page publiée le 14 mars 2022