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Instituto de Tecnológico de Estudios Superiores de Monterrey (ITESM) 2020

Analysis of microRNAs and metabolomic profiling of two different populations of Neem (Azadirachta indica A. Juss) from Mexico

Reyes Pérez, Paula Roxana

Titre : Analysis of microRNAs and metabolomic profiling of two different populations of Neem (Azadirachta indica A. Juss) from Mexico

Auteur : Reyes Pérez, Paula Roxana

Etablissement de soutenance : Instituto de Tecnológico de Estudios Superiores de Monterrey (ITESM)

Grade : Master of Science In Biotechnology 2020

Résumé partiel
Azadirachta indica, common name neem, is an evergreen tree with potential for the treatment of cardiovascular diseases, rheumatism, osteoporosis, and a mosquito repellent ; These properties are due to its rich content in phytochemicals. In Mexico, it is established in more than 12 states, but there are no previous reports of phytochemical content for Mexican neem populations. MicroRNAs (miRNAs) are highly conserved, non-coding, short RNA molecules that play important regulatory roles in plant development and metabolism, including the synthesis of secondary metabolites. The aim of the present work was to identify and characterize the miRNAs and their targets involved in the production of secondary metabolites in A. indica, employing a high stringent genome-wide computational-based approach, and following a set of strict filtering norms, followed by experimental validation. As well, to perform the metabolomic profile of two Mexican A. indica samples through gas chromatography-mass spectrometry (GC-MS) and ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-QTOF-MS). Thus, a total of 44 potential A. indica miRNAs belonging to 21 families and their corresponding 48 potential target transcripts were identified. Relevant targets included Squamosa promoter binding protein-like proteins, NAC, Scarecrow proteins, Auxin response factor, and F-box proteins. Some of the identified miRNAs showed to be involved in the biosynthesis of phenolic compounds and terpenoids. Gene network analysis was developed to understand the miRNA mediated gene regulation

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