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Sultan Qaboos University (2015)

Management of whitefly transmitted begomoviruses associated with tomato crop in Oman

Al-Shihi, Adel Ali Mohammed Hamadi.

Titre : Management of whitefly transmitted begomoviruses associated with tomato crop in Oman

Auteur : Al-Shihi, Adel Ali Mohammed Hamadi.

Université de soutenance : Sultan Qaboos University

Grade : Doctor of Philosophy in Crop Science 2015

Résumé
Tomato yellow leaf curl (TYLC) is a complex of begomovirus species which are transmitted exclusively by whitefly (Bemisia tabaci). Typical symptoms of this disease yellowing, upward leaf curling and severe stunting of the whole plant. A survey was conducted for collecting whiteflies from 2-3 farms per Wilayat from different crops. Total nucleic acid isolated from single whitefly was used as template to amplify a fragment of 220 bp from the B biotype and 400 bp for the Q biotype of the mtCOI gene of whitefly using the primers Bem23F/Bem23R. All the samples showed fragment of 200 bp indicating the presence of B biotype. Six primer pairs were used RbF/RbR (900bp), 92F/HbR (700bp), Ars23S-1/Ars23R-2 (580bp), Wol16SF/Wol16SR (650bp), CFBF/CFBR (500bp) and U23F/23SIGR (600bp) to determine the the secondary symbionts associated with whitefly Bemisia tabaci. Only a fragment of 710 bp was amplified in all the screened samples indicating the presence of Hamiltonella species. Agryl cover was used in field experiments in two successive years to evaluate the optimum period for covering the tomato plants against whitefly transmitted begomoviruses. The covering period of 6-7 weeks proved to be the best for getting the highest yield (3kg/plant) with less TYLCV infection. Tomato crops showing begomovirus-like symptoms were collected from three commercial farms at Barka, cloned and sequenced. The results showed the presence of a new begomovirus species which was named Tomato leaf curl Barka virus (TOLCBrV). Fourteen tomato inbred lines carrying Ty begomovirus resistant genes have brought from the Agricultural Vegetable Research and Development Center (AVRDC) and screened against begomoviruses under field conditions in two successive years and also under controlled conditions.

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Annonce (Almandumah)

Page publiée le 4 juin 2022