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Sultan Qaboos University (2015)

Genetic characterization of Omani cattle using microsatellite DNA markers

Al-Sinaniyah, Karima Rashed Hareb.

Titre : Genetic characterization of Omani cattle using microsatellite DNA markers

Auteur : Al-Sinaniyah, Karima Rashed Hareb.

Université de soutenance : Sultan Qaboos University

Grade : Master of Science (MS) in Animal and Veterinary Sciences 2015

Résumé partiel
The indigenous cattle breeds in Oman have special characteristics that are possibly not available for foreign breeds. They are highly preferred by consumers in Oman and they are more adapted to the harsh arid environment and prevailing diseases. A high level of cattle diversity serves the needs for designing breeding programs, increasing quality and productivity and improving gene selection programs. Because of the lack of information on genetic diversity of local cattle, the present study aimed to evaluate the genetic diversity and structure of cattle populations in Oman, using microsatellite markers. Two local Omani cattle breeds (Al-Batinah and Dhofari), two cattle breeds which are usually imported in the country (Ethiopian and Pakistani) and data generated for European breeds (Friesian, Jersey and Charolais) were included in the analyses. Blood samples were collected from 41 unrelated animals and the genomic DNA was extracted using QIAamp Blood Midi Kit. Şix microsatellite markers (recommended by the ISAG/FAO) were amplified by PCR using specified protocols. PCR products were run on agarose gel mixed with ethidium bromide then visualized under UV light. The size of microsatellite alleles were measured using 100 bp DNA ladder. Fragment analysis was carried out on ABI Prism genetic analyzer 3130. Microsatellite fragment sizing was done using the Gene Mapper program. A total of 74 alleles were distinguished by the six microsatellite primers used. The number of alleles detected per locus varied from 10 to 18, with an average of 12.3 alleles per locus. The average value for effective number of alleles over loci and population was 3.35. The level of gene diversity was high as indicated by the mean observed and expected heterozygosity values across all loci for all populations, which were 0.5890.053 and 0.719 +0.048, respectively. The mean value of polymorphic information content (PIC) was 0.815. There was a significant deviation from Hardy-Weinberg Equilibrium (HWE) at the six microsatellite loci for all populations. Significant linkage disequilibrium (p<0.05) was detected in the overall microsatellite data for 15 loci. Level of inbreeding (mean Fis = 0.098) was moderate

Présentation (SHUAA)

Annonce (Almandumah)

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