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Yarmouk University (2018)

In Vitro Antimicrobial and Other Biological Activities of Micromeria Fruticosa (L.) Druce Collected from Northern Jordan

Najadat, Irsal Mishref

Titre : In Vitro Antimicrobial and Other Biological Activities of Micromeria Fruticosa (L.) Druce Collected from Northern Jordan

دراسة تأثير نبات الزعتر الفارسي على الميكروبات والنشاطات الحيوية المختلفة له في منطقة شمال الاردن

Auteur : Najadat, Irsal Mishref

Université de soutenance : Yarmouk University

Grade : Master of Science (MS) 2018

Résumé
Micromeria fruticosa is a plant species of the Lamiaceae family. It is a medicinal shrub, native to the Mediterranean area. This study aims to assess the antimicrobial activity, antioxidant activity, phytochemicals analysis, and essential oil extraction and screening of Micromeria fruticosa from Halawah and osarah region in northern Jordan. Antimicrobial activity of methanolic and chlorformic extracts of Micromeria fruticosa were evaluated against five Gram- negative bacterial species Serratia marcescen, Escherichia coli, Proteus vulgaris, Klebsiella pneumonia, and salmonella typhimurium, and four Gram- positive bacterial species Staphylococcus aureus, Staphylococcus epidermidis, Bacillus cereus and Micrococcu luteus using agar well diffusion method and broth dilution method. Results showed that chloroformic extract was more effective against most studied bacterial species than methanolic extract. The maximum inhibition zone of (26 mm) was obtained by using Micromeria fruticosa chloroformic extract against Staphylococcus aureus, and the minimum value (10mm) was obtained by using Micromeria fruticosa methanolic extracts against Escherichia coli. The minimal inhibitory concentration (MIC) of Micromeria fruticosa were measured for tested microorganisms, and results showed that ethanolic extract of Micromeria fruticosa had MIC value ( 41.6 mg/ml) against different type of bacteria while the MIC value (37.5 mg/ml) for ethanolic extract of Micromeria fruticosa was found against Staphylococcus epidermidis. Furthermore, the stable free radical 2, 2- diphyenyl-picrylhydrazyl (DPPH) method was used to evaluate the antioxidant activity of Micromeria fruitcosa extracts. Results showed that the highest rate of radical scavenging activity (96.1%) of oxidant can be reduced or scavenge by the antioxidant was obtained from methanolic extract and the lowest rate of radical scavenging activity was recorded for chloroformic extract (21.4%). On the other hand, phytochemical analysis carried out in Micromeria fruticosa revealed the presence of flavonoid, alkaloids, glycosids, tannins, steroids, phenols and saponins in chloroformic and ethanolic extract. Using GC/MS for essential oil extracted from Micromeria fruticosa before and after defatting process, the result showed that there is (60) active compound in the essential oil and (56) in the defatted one

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Page publiée le 19 mai 2022