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Accueil du site → Master → Jordanie → Chemical Composition and Antioxidant Activity of the Essential Oil from Flower Buds and Leaves Extracts of the Cistus Creticus and Cistus Salvifolius

Yarmouk University (2017)

Chemical Composition and Antioxidant Activity of the Essential Oil from Flower Buds and Leaves Extracts of the Cistus Creticus and Cistus Salvifolius

Saleh, Noura Rebhi

Titre : Chemical Composition and Antioxidant Activity of the Essential Oil from Flower Buds and Leaves Extracts of the Cistus Creticus and Cistus Salvifolius

التركيب الكيميائي و النشاط المضاد لأكسدة للزيوت الطياره من زهور نبات اللباد الأحمر و نبات اللباد الأبيض

Auteur : Saleh, Noura Rebhi

Université de soutenance : Yarmouk University

Grade : Master of Science (MS) 2017

Résumé
The chemical composition of the volatile oils from flowers and leaves of C. creticis and C. salvifolius growing wild in Jordan and their antioxidant activities was investigated. These plants were extracted by two extraction methods, namely, hydro-distillation and solid phase micro-extraction (SPME). The SPME oil from leaves and flowers of C.creticus was dominated by monoterpenes ZLWK Į-SLQHQH Į-cubebene and camphene as major components. However in the hydro-distillation the leaves oil was dominated by sesquiterpenes and oxygenated diterpenes with manoyl oxide, sclarene DQGȖ-cadinene as major components. The flower oil dominated by oxygenated diterpenes with manoyl oxide, and abietatriene as major components. The oils from flowers and leaves of C. salvifolius obtained by SPME were dominated by monoterpenes and sesquiterpenes with ɷ-3- carene, ɲ-pinene, ɴ-pinene and E-caryophyllene as major constituents. On the other hand, the oils from flowers and leaves of C. salvifolius obtained by hydro-distillation were dominated by oxygenated diterpenes and ester with methyl strictate, abietol, methyl octadecanoate and manoyl oxide as a major component. In the leaves the major components of the oil were manoyl oxide, E-ethyl cinnamate and Z-ethyl cinnamate. Fractions of different polarities obtained from the flowers and leaves of C. creticis and C. salvifolius were screened for the presence of alkaloids, tannins, glycosides, flavonoids, saponins and terpenes. The methanolic extract of the flowers and leaves of C. creticis and C. salvifolius were partitioned in solvents of different polarities to assess their total phenol, total flavonoids content and evaluate the in vitro antioxidant activity. The in vitro antioxidant activities were examined by the DPPH and ABTS radical scavenging methods in addition to the ferrous ion chelating effect and hydroxyl radical assay methods. The results indicated that butanol and water fractions of the flowers and leaves of C. creticis and aq. methanol and butanol fractions of the flowers and leaves of C. salvifolius had a dose dependent in vitro antioxidant activity

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Page publiée le 22 avril 2022