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University of Zagreb (2013)

Effects of salinity and osmotic stress on protein differential expression in Mammillaria gracilis Pfeiff. in vitro-grown cultures

Rogić Tea

Titre : Effects of salinity and osmotic stress on protein differential expression in Mammillaria gracilis Pfeiff. in vitro-grown cultures

Utjecaj solnog i osmotskog stresa na diferencijalnu ekspresiju proteina kaktusa Mammillaria gracilis Pfeiff. u kulturi in vitro

Auteur : Rogić Tea

Université de soutenance : University of Zagreb

Grade : Master’s Thesis (magistar/magistra molekularne biologjie) 2013

Résumé
Although cacti Mammillaria gracilis Pfeiff. is not commercially interesting plant species in Europe, its adaptations on arid environments make it suitable model organism for studying biochemical and molecular mechanisms which activate during exposure of plants to salt and osmotic stress. Cacti tissues grow in vitro as habituated calli of unorganised growth capable of regeneration potential retention, and as completely unorganised tumors induced via transformation of cacti cells with Agrobacterium tumefaciens bacteria. This research showed that calli and tumor tissues respond to salt and osmotic stress at the level of soluble cell proteins synthesis. Mechanisms underlying these responses differ and are dependent of tissue used, but it seems that tumor tissue is, at least to some extent, more sensitive to impact of stress factors than calli. Differential expression protein analysis implicated heat shock protein family, as well as glyceraldehyde-3-phosphate dehydrogenase protein, as general markers of salt and osmotic stress presence in plants.

Présentation : Croatian Digital Theses Repository (CDRT)

Page publiée le 6 mai 2022