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University of Zagreb (2019)

Influence of salinity and osmotic stress on protein glycosylation in Mammillaria gracilis Pffeif.

Lambert Ines

Titre : Influence of salinity and osmotic stress on protein glycosylation in Mammillaria gracilis Pffeif.

Utjecaj solnog i osmotskog stresa na glikozilaciju proteina u kulturi tkiva kaktusa Mammillaria gracilis Pfeiff.

Auteur : Lambert Ines

Université de soutenance : University of Zagreb

Grade : Master’s Thesis (magistar/magistra edukacije biologije) 2019

Résumé
In this paper, the effects of salt and osmotic stress on proteins isolated from callus and tumour tissue lines of Mamillaria gracilis Pfeiff. cacti, grown in vitro, were compared. Because it is a plant with CAM metabolism adapted to arid habitats and sensitive to changes in environmental factors, it is a suitable model organism to study the mechanisms in plant cells that are activated during plant exposure to salt and osmotic stress. In this study, it was shown that the exposure of M. gracilis tissues to salt and mannitol alters the expression of total soluble cellular proteins and induces changes in protein N-glycosylation, a very important posttranslational modification. Osmotic stress induced by mannitol had stronger impact on callus protein N-glycosylation, while tumour tissue was found to be more sensitive to salt stress (NaCl). Difference in glycoprotein expression, which was found between callus and tumour tissue after their exposure to the same type of abiotic stress, brings to conclusion that N-glycosylation is specific to certain tissues type. On the other hand, difference in glycoprotein expression, detected within the same tissue type after exposure to different stressors (NaCl or mannitol), implies that level of protein N-glycosylation is dependent on the type of the stressor.

Présentation : Croatian Digital Theses Repository (CDRT)

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