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Kenyatta University (2021)

Phenotypic and Genetic Characterization of Selected Kenyan Vigna Radiata (l.) Wilckzek (Mung Bean) Genotypes

Wangari, Mwangi Jedidah

Titre : Phenotypic and Genetic Characterization of Selected Kenyan Vigna Radiata (l.) Wilckzek (Mung Bean) Genotypes

Auteur : Wangari, Mwangi Jedidah

Université de soutenance  : Kenyatta University

Grade : Master of Science (Biotechnology) 2021

Résumé partiel
Mung bean (Vigna radiata (L.) Wilckzeck) is a leguminous crop grown for its nutritious seeds and as a fodder crop. Characterization of mung bean germplasm is, therefore, a prerequisite for the use of its genetic resources. The objective of this study was to evaluate the phenotypic and genetic variations among seven genotypes from eastern Kenya where they are mainly grown. Phenotypic diversity was determined using 13 morphological traits done in triplicate and the data was analysed using Minitab software version 19.2. The Principal Components were also computed and Eucledian distance tool used to construct the dendogram. Two main clusters I and II were obtained with cluster I having four genotypes while cluster II comprised three genoypes. The first and the second principal component accounted for 35.6% and 29.1% phenotypic variation respectively while cumulatively they accounted for 64.7% of the total variation. Simple sequence repeat markers were used to assess the genetic diversity of the seven mung bean genotypes. A set of 10 standardized Simple Sequence Repeats markers were used and eight of them had distinct and consistent amplification profiles while two were monomorphic. Twenty three alleles were detected in all the 7 genotypes on all chromosomes after analysis. The average number of alleles per locus shown by each marker ranged from 2-5 alleles and the average was 2.875 across loci.

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Page publiée le 16 mai 2022